Increased serum interleukin-17 and peripheral Th17 cells in children with acute Henoch-Schönlein purpura

Hsiao-Yu Jen, Ya-Hui Chuang, Sheng-Chieh Lin, Bor-Luen Chiang, Yao-Hsu Yang

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45 Citations (Scopus)


Background: Interleukin (IL)-17 and Th17 cells have been involved in many autoimmune diseases. The aim of this study is to investigate the involvement of IL-17 and Th17 cells in the pathogenesis of childhood Henoch-Schönlein purpura (HSP). Methods: Serum and supernatant levels of cytokines and chemokines were analyzed by enzyme-linked immunosorbent assay (ELISA). Using intracellular staining, the frequency of peripheral Th17 and Th1 cells was studied by flow cytometry. Results: Children with acute HSP had significantly higher serum levels of IL-17, IL-6 and transforming growth factor-β than healthy controls. The IL-17 levels in culture supernatants of peripheral blood mononuclear cells with anti-CD3 and CD28 antibody stimulation were much higher in patients with HSP (281.2±91.4 vs. 47.7±22.6pg/ml, p=0.022). The patients also had more Th17 cells (1.67±0.36% vs. 0.71±0.15%, p=0.033) but not Th1 cells in peripheral blood. Moreover, IL-17 could promote human endothelial cells to produce chemoattractants IL-8 and monocyte chemotactic protein-1. Conclusion: The increased frequency of peripheral Th17 cells and serum IL-17 levels are shown in childhood HSP that may in part contribute to vascular inflammation, suggesting cellular immunity is likely to be involved in the process of HSP. © 2011 John Wiley & Sons A/S.
Original languageEnglish
Pages (from-to)862-868
Number of pages7
JournalPediatric Allergy and Immunology
Issue number8
Publication statusPublished - 2011


  • Childhood
  • Henoch-Schönlein purpura
  • Interleukin 17
  • Th17 cells
  • CD28 antigen
  • CD3 antigen
  • interleukin 17
  • interleukin 23
  • interleukin 6
  • interleukin 8
  • monocyte chemotactic protein 1
  • transforming growth factor beta
  • anaphylactoid purpura
  • article
  • blood level
  • cellular immunity
  • child
  • clinical article
  • controlled study
  • cytokine production
  • endothelium cell
  • enzyme linked immunosorbent assay
  • female
  • flow cytometry
  • human
  • human cell
  • inflammation
  • male
  • pathogenesis
  • peripheral blood mononuclear cell
  • priority journal
  • supernatant
  • Th1 cell
  • Th17 cell
  • Acute Disease
  • Adolescent
  • Cell Separation
  • Cells, Cultured
  • Chemokine CCL2
  • Child
  • Child, Preschool
  • Endothelial Cells
  • Female
  • Flow Cytometry
  • Gene Expression Regulation
  • Humans
  • Immunization
  • Interleukin-17
  • Interleukin-6
  • Male
  • Purpura, Schoenlein-Henoch
  • Th1 Cells
  • Th17 Cells
  • Transforming Growth Factor beta


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