Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis

Jehn Hsiahn Yang; Ming Yih Wu; Daw Yuan Chang; Chien Huei Chang; Yu Shih Yang; Hong Nerng Ho

doi:10.1111/j.1600-0897.2005.00363.x

Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis

Jehn Hsiahn Yang, Ming Yih Wu, Daw Yuan Chang, Chien Huei Chang, Yu Shih Yang, Hong Nerng Ho

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

Problem: To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis. Method of study: Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr. Results: Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis. Conclusion: IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.

Original language	English
Pages (from-to)	181-187
Number of pages	7
Journal	American Journal of Reproductive Immunology
Volume	55
Issue number	3
DOIs	https://doi.org/10.1111/j.1600-0897.2005.00363.x
Publication status	Published - Mar 1 2006
Externally published	Yes

Keywords

Adenomyosis
Endometrial stromal cell
Interleukin-6
Macrophage

ASJC Scopus subject areas

Immunology
Obstetrics and Gynaecology

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10.1111/j.1600-0897.2005.00363.x

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title = "Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis",

abstract = "Problem: To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis. Method of study: Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr. Results: Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis. Conclusion: IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.",

keywords = "Adenomyosis, Endometrial stromal cell, Interleukin-6, Macrophage",

author = "Yang, {Jehn Hsiahn} and Wu, {Ming Yih} and Chang, {Daw Yuan} and Chang, {Chien Huei} and Yang, {Yu Shih} and Ho, {Hong Nerng}",

year = "2006",

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doi = "10.1111/j.1600-0897.2005.00363.x",

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AU - Yang, Jehn Hsiahn

AU - Wu, Ming Yih

AU - Chang, Daw Yuan

AU - Chang, Chien Huei

AU - Yang, Yu Shih

AU - Ho, Hong Nerng

PY - 2006/3/1

Y1 - 2006/3/1

N2 - Problem: To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis. Method of study: Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr. Results: Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis. Conclusion: IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.

AB - Problem: To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis. Method of study: Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr. Results: Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis. Conclusion: IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.

KW - Adenomyosis

KW - Endometrial stromal cell

KW - Interleukin-6

KW - Macrophage

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Increased interleukin-6 messenger RNA expression in macrophage-cocultured endometrial stromal cells in adenomyosis

Abstract

Keywords

ASJC Scopus subject areas

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