Increased expression of matrix metalloproteinase-2 in oral cells after short-term stimulation and long-term usage of areca quid

Shyun Y. Liu, Mei Huei Lin, Shun Chun Yang, Guan Cheng Huang, Lisa Chang, Sam Chang, Ching Y. Yen, Wei F. Chiang, Yung Y. Kuo, Li L. Chen, Chin H. Lee, Young Chau Liu

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Background and Purpose: Arecoline, an areca quid (AQ) component, has been shown to inhibit the secretion and activity of matrix metalloproteinase-2 (MMP-2) in fibroblast cultures. This study assessed whether MMP-2 expression was inhibited in the saliva samples and tumor specimens of oral tumor patients with a long-term history of AQ consumption. The net effect of crude AQ extract (AQE) on MMP-2 expression by oral cells was also investigated. Methods: Western blot analysis, zymography, and reverse transcriptase-polymerase chain reaction were used to detect MMP-2 protein and mRNA in saliva and tumor samples, as well as in the conditioned media (CM) of oral cell cultures. Results: The level of MMP-2 protein was significantly higher in the saliva samples of 12 oral tumor patients who had a minimum 10-year AQ-consuming history than in those of 12 non-AQ-using healthy controls (p <0.05). MMP-2 mRNA was expressed in 26 of 28 oral squamous cell carcinoma (OSCC) specimens. MMP-2 protein was also detectable in the tested OSC-C homogenates. Short-term stimulation with 10% AQE increased the secretion of MMP-2 protein in the CM of oral epidermoid carcinoma cell Meng-1 (an OSCC cell line) and oral fibroblasts. Conclusions: MMP-2 expression is elevated rather than inhibited in most oral tumor patients with long-term AQ usage. Short-term AQE stimulation also increases the secretion of MMP-2 by oral epithelial cells and fibroblasts. Our results suggest that AQ consumption may promote oral tumor progression through the induction of MMP-2 secretion.

Original languageEnglish
Pages (from-to)390-397
Number of pages8
JournalJournal of the Formosan Medical Association
Issue number6
Publication statusPublished - 2005
Externally publishedYes


  • Areca
  • Carcinoma
  • Fibroblasts
  • Matrix metalloproteinases
  • Squamous cell

ASJC Scopus subject areas

  • General Medicine


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