Increase of cardiac M2-muscarinic receptor gene expression in type-1 but not in type-2 diabetic rats

Liang Ming Lee; Cheng Kuei Chang; Kai Chun Cheng; Dai Huang Kou; I. Min Liu; Juei Tang Cheng

doi:10.1016/j.neulet.2008.06.003

Increase of cardiac M₂-muscarinic receptor gene expression in type-1 but not in type-2 diabetic rats

Liang Ming Lee
, Cheng Kuei Chang
, Kai Chun Cheng
, Dai Huang Kou
, I. Min Liu
, Juei Tang Cheng

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Changes of cardiac M₂-muscarinic receptor (M₂-mAChR) gene expression was investigated in type-1 like diabetic rats induced by intravenous injection of streptozotocin (STZ) and type-2 like diabetic rats induced by fed with fructose-rich chow. Systolic blood pressure (SBP) in STZ-diabetic rats was significantly lower than that in age-matched non-diabetic rats, while the SBP in type-2 like diabetic rats was higher than in non-diabetic rats. Also, the mRNA or protein level of cardiac M₂-mAChR in STZ-diabetic rats was markedly higher than non-diabetic rats, but it was not observed in type-2 like diabetic rats as compared to age-matched non-diabetic rats. Arecaidine propargyl ester (APE), the agonist of M₂-mAChR, produced a marked reduction of heart rate in STZ-diabetic rats but made less influence on heart rate in fructose-fed rats or non-diabetic rats. The results suggest that cardiac M₂-mAChR gene expression is raised in type-1 like diabetic rats but not in type-2 like diabetic rats, this difference mainly due to hyperglycemia, for the production of hypotension in diabetic disorders.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Neuroscience Letters
Volume	441
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2008.06.003
Publication status	Published - Aug 22 2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cardiac M-muscarinic receptor
Gene expression
Hyperglycemia
Insulin resistance

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/j.neulet.2008.06.003

Cite this

@article{b8138dd2defe4d4192c9ae253b4370a9,

title = "Increase of cardiac M2-muscarinic receptor gene expression in type-1 but not in type-2 diabetic rats",

abstract = "Changes of cardiac M2-muscarinic receptor (M2-mAChR) gene expression was investigated in type-1 like diabetic rats induced by intravenous injection of streptozotocin (STZ) and type-2 like diabetic rats induced by fed with fructose-rich chow. Systolic blood pressure (SBP) in STZ-diabetic rats was significantly lower than that in age-matched non-diabetic rats, while the SBP in type-2 like diabetic rats was higher than in non-diabetic rats. Also, the mRNA or protein level of cardiac M2-mAChR in STZ-diabetic rats was markedly higher than non-diabetic rats, but it was not observed in type-2 like diabetic rats as compared to age-matched non-diabetic rats. Arecaidine propargyl ester (APE), the agonist of M2-mAChR, produced a marked reduction of heart rate in STZ-diabetic rats but made less influence on heart rate in fructose-fed rats or non-diabetic rats. The results suggest that cardiac M2-mAChR gene expression is raised in type-1 like diabetic rats but not in type-2 like diabetic rats, this difference mainly due to hyperglycemia, for the production of hypotension in diabetic disorders.",

keywords = "Cardiac M-muscarinic receptor, Gene expression, Hyperglycemia, Insulin resistance",

author = "Lee, \{Liang Ming\} and Chang, \{Cheng Kuei\} and Cheng, \{Kai Chun\} and Kou, \{Dai Huang\} and Liu, \{I. Min\} and Cheng, \{Juei Tang\}",

note = "Funding Information: We appreciate the kind assistance of Miss F.Y. Jou in blotting analysis. Thanks are also due to Professor S.S. Liu for generously supplying the plasmids containing the cDNA of β-actin. The present study was supported in part by a grant from the National Science Council (NSC 94-2320-B-006-089) of the Republic of China. ",

year = "2008",

month = aug,

day = "22",

doi = "10.1016/j.neulet.2008.06.003",

language = "English",

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journal = "Neuroscience Letters",

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T1 - Increase of cardiac M2-muscarinic receptor gene expression in type-1 but not in type-2 diabetic rats

AU - Lee, Liang Ming

AU - Chang, Cheng Kuei

AU - Cheng, Kai Chun

AU - Kou, Dai Huang

AU - Liu, I. Min

AU - Cheng, Juei Tang

N1 - Funding Information: We appreciate the kind assistance of Miss F.Y. Jou in blotting analysis. Thanks are also due to Professor S.S. Liu for generously supplying the plasmids containing the cDNA of β-actin. The present study was supported in part by a grant from the National Science Council (NSC 94-2320-B-006-089) of the Republic of China.

PY - 2008/8/22

Y1 - 2008/8/22

N2 - Changes of cardiac M2-muscarinic receptor (M2-mAChR) gene expression was investigated in type-1 like diabetic rats induced by intravenous injection of streptozotocin (STZ) and type-2 like diabetic rats induced by fed with fructose-rich chow. Systolic blood pressure (SBP) in STZ-diabetic rats was significantly lower than that in age-matched non-diabetic rats, while the SBP in type-2 like diabetic rats was higher than in non-diabetic rats. Also, the mRNA or protein level of cardiac M2-mAChR in STZ-diabetic rats was markedly higher than non-diabetic rats, but it was not observed in type-2 like diabetic rats as compared to age-matched non-diabetic rats. Arecaidine propargyl ester (APE), the agonist of M2-mAChR, produced a marked reduction of heart rate in STZ-diabetic rats but made less influence on heart rate in fructose-fed rats or non-diabetic rats. The results suggest that cardiac M2-mAChR gene expression is raised in type-1 like diabetic rats but not in type-2 like diabetic rats, this difference mainly due to hyperglycemia, for the production of hypotension in diabetic disorders.

AB - Changes of cardiac M2-muscarinic receptor (M2-mAChR) gene expression was investigated in type-1 like diabetic rats induced by intravenous injection of streptozotocin (STZ) and type-2 like diabetic rats induced by fed with fructose-rich chow. Systolic blood pressure (SBP) in STZ-diabetic rats was significantly lower than that in age-matched non-diabetic rats, while the SBP in type-2 like diabetic rats was higher than in non-diabetic rats. Also, the mRNA or protein level of cardiac M2-mAChR in STZ-diabetic rats was markedly higher than non-diabetic rats, but it was not observed in type-2 like diabetic rats as compared to age-matched non-diabetic rats. Arecaidine propargyl ester (APE), the agonist of M2-mAChR, produced a marked reduction of heart rate in STZ-diabetic rats but made less influence on heart rate in fructose-fed rats or non-diabetic rats. The results suggest that cardiac M2-mAChR gene expression is raised in type-1 like diabetic rats but not in type-2 like diabetic rats, this difference mainly due to hyperglycemia, for the production of hypotension in diabetic disorders.

KW - Cardiac M-muscarinic receptor

KW - Gene expression

KW - Hyperglycemia

KW - Insulin resistance

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