In vivo fate mapping of cryopreserved murine ovarian grafts

Chi Huang Chen; Shun Jen Tan; Chii Ruey Tzeng

doi:10.1186/s13048-014-0081-7

In vivo fate mapping of cryopreserved murine ovarian grafts

Chi Huang Chen, Shun Jen Tan, Chii Ruey Tzeng

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Background: Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Methods: Ovaries of donor FVB/N-Tg (PolII-Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts. Results: There were significantly stronger BLI signals in the fresh ovaries than in the frozen-thawed ones. The number of primordial follicles was significantly lower in frozen-thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen-thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation. Conclusions: Ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.

Original language	English
Article number	81
Journal	Journal of Ovarian Research
Volume	7
Issue number	1
DOIs	https://doi.org/10.1186/s13048-014-0081-7
Publication status	Published - Aug 15 2014

Keywords

AMH
Bioluminescence imaging
Cryopreservation
Ovary
Transgenic

ASJC Scopus subject areas

Oncology
Obstetrics and Gynaecology

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10.1186/s13048-014-0081-7

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title = "In vivo fate mapping of cryopreserved murine ovarian grafts",

abstract = "Background: Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Methods: Ovaries of donor FVB/N-Tg (PolII-Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts. Results: There were significantly stronger BLI signals in the fresh ovaries than in the frozen-thawed ones. The number of primordial follicles was significantly lower in frozen-thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen-thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation. Conclusions: Ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.",

keywords = "AMH, Bioluminescence imaging, Cryopreservation, Ovary, Transgenic",

author = "Chen, {Chi Huang} and Tan, {Shun Jen} and Tzeng, {Chii Ruey}",

note = "Funding Information: This work was supported by grant NSC 97-2314-B-016 -012 -MY2 from National Science Council, Taiwan, Republic of China. Publisher Copyright: {\textcopyright} 2014 Chen et al.; licensee BioMed Central Ltd.",

year = "2014",

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doi = "10.1186/s13048-014-0081-7",

language = "English",

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AU - Chen, Chi Huang

AU - Tan, Shun Jen

AU - Tzeng, Chii Ruey

N1 - Funding Information: This work was supported by grant NSC 97-2314-B-016 -012 -MY2 from National Science Council, Taiwan, Republic of China. Publisher Copyright: © 2014 Chen et al.; licensee BioMed Central Ltd.

PY - 2014/8/15

Y1 - 2014/8/15

N2 - Background: Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Methods: Ovaries of donor FVB/N-Tg (PolII-Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts. Results: There were significantly stronger BLI signals in the fresh ovaries than in the frozen-thawed ones. The number of primordial follicles was significantly lower in frozen-thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen-thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation. Conclusions: Ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.

AB - Background: Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy. Methods: Ovaries of donor FVB/N-Tg (PolII-Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts. Results: There were significantly stronger BLI signals in the fresh ovaries than in the frozen-thawed ones. The number of primordial follicles was significantly lower in frozen-thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen-thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation. Conclusions: Ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.

KW - AMH

KW - Bioluminescence imaging

KW - Cryopreservation

KW - Ovary

KW - Transgenic

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In vivo fate mapping of cryopreserved murine ovarian grafts

Abstract

Keywords

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