In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy

Hsiung Lin Tu; Yu Shen Lin; Hsia Yu Lin; Yann Hung; Leu Wei Lo; Yang Fang Chen; Chung Yuan Mou

doi:10.1002/adma.200800548

In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy

Hsiung Lin Tu, Yu Shen Lin, Hsia Yu Lin, Yann Hung, Leu Wei Lo, Yang Fang Chen, Chung Yuan Mou

Research output: Contribution to journal › Article › peer-review

198 Citations (Scopus)

Abstract

A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.

Original language	English
Pages (from-to)	172-177
Number of pages	6
Journal	Advanced Materials
Volume	21
Issue number	2
DOIs	https://doi.org/10.1002/adma.200800548
Publication status	Published - Jan 12 2009
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Mechanics of Materials
Mechanical Engineering

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title = "In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy",

abstract = "A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.",

author = "Tu, {Hsiung Lin} and Lin, {Yu Shen} and Lin, {Hsia Yu} and Yann Hung and Lo, {Leu Wei} and Chen, {Yang Fang} and Mou, {Chung Yuan}",

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AU - Tu, Hsiung Lin

AU - Lin, Yu Shen

AU - Lin, Hsia Yu

AU - Hung, Yann

AU - Lo, Leu Wei

AU - Chen, Yang Fang

AU - Mou, Chung Yuan

PY - 2009/1/12

Y1 - 2009/1/12

N2 - A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.

AB - A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.

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In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy

Abstract

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