In vitro studies of functionalized mesoporous silica nanoparticles for photodynamic therapy

Hsiung Lin Tu, Yu Shen Lin, Hsia Yu Lin, Yann Hung, Leu Wei Lo, Yang Fang Chen, Chung Yuan Mou

Research output: Contribution to journalArticlepeer-review

192 Citations (Scopus)


A simple surface modification process that conjugates a photosensitizer, photoporphyrin IX (PpIX), with mesoporous silica nanoparticles (MSNs) through covalent bonding to yield PpIX-modified for photodynamic therapy (PDT) study, was reported. In-vitro tests performed with HeLa cells revealed high cellular uptake efficiency and the phototoxicity was found to be both irradiation time and dosage dependent. Well-dispersed MSNs were synthesized in ammonium solution containing dilute tetraethyl orthosilicate (TEOS) and a low surfactant concentration. The surfactant templates were removed by the extraction in acidic ethanol to obtain surfactant-free MSNs. Amine-functionalized MSNs were obtained by post-modification of Ext-MSNs with 3-aminopropyl-trimethoxysilane (APTMS). The amine group then conjugated with activated PpIX to form PpIX-MSNs. Elemental analysis and the UV/Vis absorption spectrum were used to estimate the concentration of PpIX on PpIX-MSNs. The surface area, pore size, and pore volume were all decreased with APTMS and PpIX.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalAdvanced Materials
Issue number2
Publication statusPublished - Jan 12 2009
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering


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