Cuphiin D1 (CD1), a macrocyclic hydrolyzable tannin isolated from Cuphea hyssopifolia, has been shown to exert antitumor activity both in vitro and in vivo. Moreover, the antitumor effects of CD1 are not only related to its cytotoxicity to carcinoma cell lines, but also depend on host-mediated mechanisms. In the present study, CD1 was investigated for its effects on the proliferation and cytokine secretion of human peripheral blood mononuclear cells (PBMCs). At concentrations of from 6.25 to 50 μg/ml, it enhanced the 3H-thymidine incorporation of concanavalin A (Con A)-stimulated PBMCs in a dose-dependent manner. Excretion of IL-1β, IL-2 and TNF-α by CD1-stimulated PBMCs was markedly increased in a dose-dependent manner. The results show that CD1 could stimulate PBMCs release of IL-1β, IL-2 and TNF-α and then activate T cells. Therefore, CD1-activated T cells via IL-1β in vitro might account for the host-mediated CD1 mechanism of action.

Original languageEnglish
Pages (from-to)4233-4236
Number of pages4
JournalAnticancer Research
Issue number6 C
Publication statusPublished - Nov 2002


  • Cuphea hyssopifolia
  • Cuphiin D1
  • Human peripheral blood mononuclear cells
  • IL-1β
  • Macrocyclic hydrolyzable tannin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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