In vitro cell behavior of osteoblasts on pyrost bone substitute

Yang Hwei Tsuang, Feng Huei Lin, Jui Sheng Sun, Yi Shiong Hang, Hwa Chang Liu

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute. Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope. Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes. Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalAnatomical Record
Issue number2
Publication statusPublished - Feb 1997
Externally publishedYes


  • adhesion
  • osteoblasts
  • pyrost bone
  • spreading

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)


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