In Vitro Antimicrobial Potential of CAPE and Caffeamide Derivatives against Oral Microbes

Yin Hwa Shih, Shih Min Hsia, Kuo Chou Chiu, Tong Hong Wang, Chi Ying Chien, Po Jung Li, Yueh Hsiung Kuo, Tzong Ming Shieh

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Caffeic acid phenethyl ester (CAPE) is a natural component isolated from propolis and used in traditional medicine. We aimed to investigate the antimicrobial properties and action mechanism of CAPE and caffeamide derivatives (26G and 36M) against oral disease microbes. We resolved the minimum inhibitory and bactericidal concentrations of 26G and 36M and their stability at different temperatures and pH. We also evaluated their effect on biofilm formation and antibiotic resistance gene expression in methicillin-resistant Staphylococcus aureus (MRSA). Our results revealed that 26G and 36M showed the best anticancer and antimicrobial activities, respectively, compared with the other four caffeamide derivatives. Both 26G and 36M showed heat-dependent decreases in antimicrobial activity. The 36M derivative was stable irrespective of pH, whereas 26G was not stable under high pH conditions. Biofilm formation and antibiotic resistance-related gene expression were consistent with their respective phenotypes. This study provides evidence for the potential application of CAPE and caffeamide derivatives in dental medicine to cure or prevent oral diseases.

Original languageEnglish
Article number4099
JournalInternational journal of molecular sciences
Issue number8
Publication statusPublished - Apr 2022


  • antibiotic resistance
  • biofilm
  • caffeamide
  • caffeic acid phenethyl ester (CAPE)
  • minimum bactericidal concentration
  • minimum inhibitory concentration

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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