Improvement in resolution of laser capture microdissection using near-field probe to capture nanoparticles

Chien Ming Chen, Jen Ai Lee, Chi Fu Yen

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Purpose: A modified laser capture microdissection (LCM) system is developed to improve resolution to 400 nm, using a laser light (808 nm) transmitted by a near-field tip probe. Materials and methods: Using a 150-nm aperture to heat an ethylene vinyl acetate (EVA) film, melted spots on the average of 400 nm in diameter are generated on the underlying target composed of a 20-nm gold-particle monolayer. The near-field tip probe composed of fiber is set on a 2-D nanometer piezoactuator (PZT) for precise capturing of the monolayer of gold particles.The monolayer of gold particles under the target is bound to the EVA film using a laser, while the remaining EVA film stays on the monolayer. Results: The diameter of the melted spots as small as 400 nm are produced and details are provided that demonstrate the feasibility of the nanooperation of this new LCMsystem. Conclusion: The new LCMsystem successfully captures nanoparticles and improves resolution of microdissection to 400 nm. With this LCM system, the isolation of a single organelle or bacterium is possible.

Original languageEnglish
Article number4806083
Pages (from-to)113-119
Number of pages7
JournalIEEE Transactions on Nanobioscience
Issue number2
Publication statusPublished - Jun 2009


  • Laser capturemicrodissection (LCM)
  • Nanooperation
  • Near-field fiber probe

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Medicine (miscellaneous)
  • Bioengineering
  • Computer Science Applications
  • Biotechnology
  • Biomedical Engineering
  • Electrical and Electronic Engineering


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