TY - JOUR
T1 - Improved gene expression by a U3-based retroviral vector
AU - Chen, Bing Fang
AU - Hsieh, Chia Ling
AU - Chen, Ding Shinn
AU - Hwang, Lih Hwa
N1 - Funding Information:
We want to thank Dr. Lau, C. F. for carefully reading this manuscript. Part of this work was done at Development Center for Biotechnology (DCB) and we greatly thank DCB for providing us excellent environments. The work was supported by National Science Council of Taiwan from grant NSC 80-0418-B-169-01.
PY - 1992/4/15
Y1 - 1992/4/15
N2 - To improve the expression of the genes transduced by retroviral vectors, we have constructed a U3-based retroviral vector and evaluated its effect on the expression of an insert from the internal promoter. The unique feature of the vector is that the transduced gene is inserted at the U3 region of the 3′ long terminal repeats (LTR). Consequently, in the infected cells the gene is duplicated and transferred to the 5′-LTR. When compared with the conventional retroviral vectors which insert the gene within the retroviral transcriptional unit, the U3-based vectors greatly enhanced the expression of the transduced gene under all three promoters tested, viz. the cytomegalovirus immediately early gene promoter (CMV), the SV40 early gene promoter (SV), and the herpes simplex virus thymidine kinase gene promoter (TK). The SV and TK promoters which were previously shown suppressed by the retroviral promoter in the conventional construction restored their potencies in the U3-based vectors. Our results therefore suggested that the U3-based vectors are more advantageous than the conventional vectors for gene expression.
AB - To improve the expression of the genes transduced by retroviral vectors, we have constructed a U3-based retroviral vector and evaluated its effect on the expression of an insert from the internal promoter. The unique feature of the vector is that the transduced gene is inserted at the U3 region of the 3′ long terminal repeats (LTR). Consequently, in the infected cells the gene is duplicated and transferred to the 5′-LTR. When compared with the conventional retroviral vectors which insert the gene within the retroviral transcriptional unit, the U3-based vectors greatly enhanced the expression of the transduced gene under all three promoters tested, viz. the cytomegalovirus immediately early gene promoter (CMV), the SV40 early gene promoter (SV), and the herpes simplex virus thymidine kinase gene promoter (TK). The SV and TK promoters which were previously shown suppressed by the retroviral promoter in the conventional construction restored their potencies in the U3-based vectors. Our results therefore suggested that the U3-based vectors are more advantageous than the conventional vectors for gene expression.
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U2 - 10.1016/0006-291X(92)91197-X
DO - 10.1016/0006-291X(92)91197-X
M3 - Article
C2 - 1567440
AN - SCOPUS:0026750252
SN - 0006-291X
VL - 184
SP - 330
EP - 337
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -