Improved CaP Nanoparticles for Nucleic Acid and Protein Delivery to Neural Primary Cultures and Stem Cells

Yu Wen Chao, Yen Lurk Lee, Ching San Tseng, Lily Ueh Hsi Wang, Kuo Chiang Hsia, Huatao Chen, Jean Michel Fustin, Sayma Azeem, Tzu Tung Chang, Chiung Ya Chen, Fan Che Kung, Yi Ping Hsueh, Yi Shuian Huang, Hsu Wen Chao

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Efficiently delivering exogenous materials into primary neurons and neural stem cells (NSCs) has long been a challenge in neurobiology. Existing methods have struggled with complex protocols, unreliable reproducibility, high immunogenicity, and cytotoxicity, causing a huge conundrum and hindering in-depth analyses. Here, we establish a cutting-edge method for transfecting primary neurons and NSCs, named teleofection, by a two-step process to enhance the formation of biocompatible calcium phosphate (CaP) nanoparticles. Teleofection enables both nucleic acid and protein transfection into primary neurons and NSCs, eliminating the need for specialized skills and equipment. It can easily fine-tune transfection efficiency by adjusting the incubation time and nanoparticle quantity, catering to various experimental requirements. Teleofection’s versatility allows for the delivery of different cargos into the same cell culture, whether simultaneously or sequentially. This flexibility proves invaluable for long-term studies, enabling the monitoring of neural development and synapse plasticity. Moreover, teleofection ensures the consistent and robust expression of delivered genes, facilitating molecular and biochemical investigations. Teleofection represents a significant advancement in neurobiology, which has promise to transcend the limitations of current gene delivery methods. It offers a user-friendly, cost-effective, and reproducible approach for researchers, potentially revolutionizing our understanding of brain function and development.

Original languageEnglish
Pages (from-to)4822-4839
Number of pages18
JournalACS Nano
Issue number6
Publication statusPublished - Feb 13 2024


  • calcium phosphate
  • gene delivery
  • nanoparticles
  • neural stem cells
  • primary neurons
  • transfection

ASJC Scopus subject areas

  • General Materials Science
  • General Engineering
  • General Physics and Astronomy


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