Immunohistochemical localization of D-aspartate in the rat pineal gland

Jen Ai Lee; Hiroshi Homma; Kumiko Sakai; Takeshi Fukushima; Tomofumi Santa; Ken Tashiro; Takeshi Iwatsubo; Masayoshi Yoshikawa; Kazuhiro Imai

doi:10.1006/bbrc.1996.5902

Immunohistochemical localization of D-aspartate in the rat pineal gland

Jen Ai Lee
, Hiroshi Homma
, Kumiko Sakai
, Takeshi Fukushima
, Tomofumi Santa
, Ken Tashiro
, Takeshi Iwatsubo
, Masayoshi Yoshikawa
, Kazuhiro Imai

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

Abstract

Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

Original language	English
Pages (from-to)	505-508
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	231
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.5902
Publication status	Published - Feb 13 1997
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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abstract = "Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.",

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AU - Lee, Jen Ai

AU - Homma, Hiroshi

AU - Sakai, Kumiko

AU - Fukushima, Takeshi

AU - Santa, Tomofumi

AU - Tashiro, Ken

AU - Iwatsubo, Takeshi

AU - Yoshikawa, Masayoshi

AU - Imai, Kazuhiro

PY - 1997/2/13

Y1 - 1997/2/13

N2 - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

AB - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

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Immunohistochemical localization of D-aspartate in the rat pineal gland

Abstract

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