TY - JOUR
T1 - Immunohistochemical localization of D-aspartate in the rat pineal gland
AU - Lee, Jen Ai
AU - Homma, Hiroshi
AU - Sakai, Kumiko
AU - Fukushima, Takeshi
AU - Santa, Tomofumi
AU - Tashiro, Ken
AU - Iwatsubo, Takeshi
AU - Yoshikawa, Masayoshi
AU - Imai, Kazuhiro
PY - 1997/2/13
Y1 - 1997/2/13
N2 - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.
AB - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.
UR - http://www.scopus.com/inward/record.url?scp=0031566243&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031566243&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.5902
DO - 10.1006/bbrc.1996.5902
M3 - Article
C2 - 9070309
AN - SCOPUS:0031566243
SN - 0006-291X
VL - 231
SP - 505
EP - 508
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -