Immunohistochemical localization of D-aspartate in the rat pineal gland

Jen Ai Lee; Hiroshi Homma; Kumiko Sakai; Takeshi Fukushima; Tomofumi Santa; Ken Tashiro; Takeshi Iwatsubo; Masayoshi Yoshikawa; Kazuhiro Imai

doi:10.1006/bbrc.1996.5902

Immunohistochemical localization of D-aspartate in the rat pineal gland

Jen Ai Lee, Hiroshi Homma, Kumiko Sakai, Takeshi Fukushima, Tomofumi Santa, Ken Tashiro, Takeshi Iwatsubo, Masayoshi Yoshikawa, Kazuhiro Imai

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

Original language	English
Pages (from-to)	505-508
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	231
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1996.5902
Publication status	Published - Feb 13 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1006/bbrc.1996.5902

Cite this

@article{bb0219f040d84cb991c5573b4cac13fe,

title = "Immunohistochemical localization of D-aspartate in the rat pineal gland",

abstract = "Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.",

author = "Lee, {Jen Ai} and Hiroshi Homma and Kumiko Sakai and Takeshi Fukushima and Tomofumi Santa and Ken Tashiro and Takeshi Iwatsubo and Masayoshi Yoshikawa and Kazuhiro Imai",

year = "1997",

month = feb,

day = "13",

doi = "10.1006/bbrc.1996.5902",

language = "English",

volume = "231",

pages = "505--508",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Immunohistochemical localization of D-aspartate in the rat pineal gland

AU - Lee, Jen Ai

AU - Homma, Hiroshi

AU - Sakai, Kumiko

AU - Fukushima, Takeshi

AU - Santa, Tomofumi

AU - Tashiro, Ken

AU - Iwatsubo, Takeshi

AU - Yoshikawa, Masayoshi

AU - Imai, Kazuhiro

PY - 1997/2/13

Y1 - 1997/2/13

N2 - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

AB - Specific polyclonal antibody was raised against D-aspartate (D-Asp) which had been conjugated to glutaraldehyde and was purified by affinity chromatography. Immunohistochemical staining of rat pineal gland with the antibody demonstrated the presence of D-Asp in the cytoplasm of pinealocytes, the predominant cell type in this gland. D-Asp immunoreactivity was more evident in the distal region than in the proximal region of the gland. Pinealocytes in the distal region are presumably involved in the synthesis and secretion of the pineal hormone, melatonin, and the results of staining may indicate some yet unknown role of D-Asp in the regulation of melatonin secretion.

UR - http://www.scopus.com/inward/record.url?scp=0031566243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031566243&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.5902

DO - 10.1006/bbrc.1996.5902

M3 - Article

C2 - 9070309

AN - SCOPUS:0031566243

SN - 0006-291X

VL - 231

SP - 505

EP - 508

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Immunohistochemical localization of D-aspartate in the rat pineal gland

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this