Immune response to respiratory syncytial virus: Prevention of syncytia formation by human serum during in Vitro infection

H. Faden; Tzou-Yien Lin; J. J. Hong; P. L. Ogra

doi:10.1203/00006450-198410000-00016

Immune response to respiratory syncytial virus: Prevention of syncytia formation by human serum during in Vitro infection

H. Faden, Tzou-Yien Lin, J. J. Hong, P. L. Ogra

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Abstract

Human serum specimens containing respiratory syncytial virus (RSV)–specific neutralizing antibody were found to prevent the formation of syncytia when applied to HEp–2 tissue culture monolayers which had been infected with RSV 12 h previously. This was evidenced by the demonstration of RSV–infected cells without any syncytia formation in the monolayers treated with RSV antibody–positive serum. On the other hand, widespread syncytia formation was observed with antibody–negative control serum. The inhibitory effects of RSV antibody progressively declined when applied beyond 12 h after infection. Protection of the monolayer against syncytia formation occurred only in the presence of antibody and was quickly lost after the serum was removed. The titer of antisyncytial antibody correlated with the titer of neurtralization antibody.

Original language	English
Pages (from-to)	987-991
Number of pages	5
Journal	Pediatric Research
Volume	18
Issue number	10
DOIs	https://doi.org/10.1203/00006450-198410000-00016
Publication status	Published - Jan 1 1984

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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title = "Immune response to respiratory syncytial virus: Prevention of syncytia formation by human serum during in Vitro infection",

abstract = "Human serum specimens containing respiratory syncytial virus (RSV)–specific neutralizing antibody were found to prevent the formation of syncytia when applied to HEp–2 tissue culture monolayers which had been infected with RSV 12 h previously. This was evidenced by the demonstration of RSV–infected cells without any syncytia formation in the monolayers treated with RSV antibody–positive serum. On the other hand, widespread syncytia formation was observed with antibody–negative control serum. The inhibitory effects of RSV antibody progressively declined when applied beyond 12 h after infection. Protection of the monolayer against syncytia formation occurred only in the presence of antibody and was quickly lost after the serum was removed. The titer of antisyncytial antibody correlated with the titer of neurtralization antibody.",

author = "H. Faden and Tzou-Yien Lin and Hong, {J. J.} and Ogra, {P. L.}",

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T1 - Immune response to respiratory syncytial virus

T2 - Prevention of syncytia formation by human serum during in Vitro infection

AU - Faden, H.

AU - Lin, Tzou-Yien

AU - Hong, J. J.

AU - Ogra, P. L.

PY - 1984/1/1

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N2 - Human serum specimens containing respiratory syncytial virus (RSV)–specific neutralizing antibody were found to prevent the formation of syncytia when applied to HEp–2 tissue culture monolayers which had been infected with RSV 12 h previously. This was evidenced by the demonstration of RSV–infected cells without any syncytia formation in the monolayers treated with RSV antibody–positive serum. On the other hand, widespread syncytia formation was observed with antibody–negative control serum. The inhibitory effects of RSV antibody progressively declined when applied beyond 12 h after infection. Protection of the monolayer against syncytia formation occurred only in the presence of antibody and was quickly lost after the serum was removed. The titer of antisyncytial antibody correlated with the titer of neurtralization antibody.

AB - Human serum specimens containing respiratory syncytial virus (RSV)–specific neutralizing antibody were found to prevent the formation of syncytia when applied to HEp–2 tissue culture monolayers which had been infected with RSV 12 h previously. This was evidenced by the demonstration of RSV–infected cells without any syncytia formation in the monolayers treated with RSV antibody–positive serum. On the other hand, widespread syncytia formation was observed with antibody–negative control serum. The inhibitory effects of RSV antibody progressively declined when applied beyond 12 h after infection. Protection of the monolayer against syncytia formation occurred only in the presence of antibody and was quickly lost after the serum was removed. The titer of antisyncytial antibody correlated with the titer of neurtralization antibody.

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Immune response to respiratory syncytial virus: Prevention of syncytia formation by human serum during in Vitro infection

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