Imaging receptor microdomains on leukocyte subsets in live mice

Elaine Y. Chiang; Andrés Hidalgo; Jungshan Chang; Paul S. Frenette

doi:10.1038/nmeth1018

Imaging receptor microdomains on leukocyte subsets in live mice

Elaine Y. Chiang, Andrés Hidalgo, Jungshan Chang, Paul S. Frenette

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45⁺ F4/80^- Gr-1⁺ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45⁺ B220⁺ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1⁺ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

Original language	English
Pages (from-to)	219-222
Number of pages	4
Journal	Nature Methods
Volume	4
Issue number	3
DOIs	https://doi.org/10.1038/nmeth1018
Publication status	Published - Mar 2007
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth1018

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AU - Hidalgo, Andrés

AU - Chang, Jungshan

AU - Frenette, Paul S.

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AB - We present a simple method to identify the recruitment of leukocyte subsets and determine concurrent surface-receptor clustering in live mice. We show that CD45+ F4/80- Gr-1+ neutrophils are robustly recruited in surgery-activated cremasteric venules, whereas adherent CD45+ B220+ B lymphocytes were dominant in bone marrow venules. Most adherent Gr-1+ leukocytes are not firmly stationary but actively migrate on TNF-α-activated cremasteric venular endothelium and exhibit marked polarization of surface PSGL-1, but not LFA-1, to the trailing edge.

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Imaging receptor microdomains on leukocyte subsets in live mice

Abstract

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