Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

Lun De Liao; Meng Lin Li; Hsin Yi Lai; Yen Yu I Shih; Yu Chun Lo; Siny Tsang; Paul Chang Po Chao; Chin Teng Lin; Fu Shan Jaw; You Yin Chen

doi:10.1016/j.neuroimage.2010.03.065

Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

Lun De Liao, Meng Lin Li, Hsin Yi Lai, Yen Yu I Shih, Yu Chun Lo, Siny Tsang, Paul Chang Po Chao, Chin Teng Lin, Fu Shan Jaw, You Yin Chen

Research output: Contribution to journal › Article › peer-review

116 Citations (Scopus)

Abstract

The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO₂). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ₅₇₀), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO₂ changes were sensed by pixel-wise normalization of images acquired at Λ₅₆₀ or Λ₆₀₀ to those at Λ₅₇₀. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO₂ and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.

Original language	English
Pages (from-to)	562-570
Number of pages	9
Journal	NeuroImage
Volume	52
Issue number	2
DOIs	https://doi.org/10.1016/j.neuroimage.2010.03.065
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

Electrical stimulation
Functional photoacoustic microscopy (fPAM)
Hemoglobin oxygen saturation (SO)
Total hemoglobin concentration (HbT)

ASJC Scopus subject areas

Cognitive Neuroscience
Neurology
General Medicine

Access to Document

10.1016/j.neuroimage.2010.03.065

Cite this

@article{ce2bcdc58e184fdb967c1052c3b73a3f,

title = "Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy",

abstract = "The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ570), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at Λ560 or Λ600 to those at Λ570. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.",

keywords = "Electrical stimulation, Functional photoacoustic microscopy (fPAM), Hemoglobin oxygen saturation (SO), Total hemoglobin concentration (HbT)",

author = "Liao, {Lun De} and Li, {Meng Lin} and Lai, {Hsin Yi} and Shih, {Yen Yu I} and Lo, {Yu Chun} and Siny Tsang and Chao, {Paul Chang Po} and Lin, {Chin Teng} and Jaw, {Fu Shan} and Chen, {You Yin}",

note = "Funding Information: The authors are grateful to Po-Hsun Wang for his prior system development. We are greatly indebted to the National Science Council of Taiwan, R.O.C . for the support of this research through shares in grant nos. NSC 96-2220-E-009-029 , NSC 97-2220-E-009-029, and NSC 97-2221-E-007-084-MY3 . Furthermore, this work was supported in part by the Top University Program of MOE, Taiwan, R.O.C. and the Diamond Project of NCTU . Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "2010",

month = aug,

doi = "10.1016/j.neuroimage.2010.03.065",

language = "English",

volume = "52",

pages = "562--570",

journal = "NeuroImage",

issn = "1053-8119",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

AU - Liao, Lun De

AU - Li, Meng Lin

AU - Lai, Hsin Yi

AU - Shih, Yen Yu I

AU - Lo, Yu Chun

AU - Tsang, Siny

AU - Chao, Paul Chang Po

AU - Lin, Chin Teng

AU - Jaw, Fu Shan

AU - Chen, You Yin

N1 - Funding Information: The authors are grateful to Po-Hsun Wang for his prior system development. We are greatly indebted to the National Science Council of Taiwan, R.O.C . for the support of this research through shares in grant nos. NSC 96-2220-E-009-029 , NSC 97-2220-E-009-029, and NSC 97-2221-E-007-084-MY3 . Furthermore, this work was supported in part by the Top University Program of MOE, Taiwan, R.O.C. and the Diamond Project of NCTU . Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2010/8

Y1 - 2010/8

N2 - The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ570), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at Λ560 or Λ600 to those at Λ570. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.

AB - The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ570), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at Λ560 or Λ600 to those at Λ570. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.

KW - Electrical stimulation

KW - Functional photoacoustic microscopy (fPAM)

KW - Hemoglobin oxygen saturation (SO)

KW - Total hemoglobin concentration (HbT)

UR - http://www.scopus.com/inward/record.url?scp=77953718613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953718613&partnerID=8YFLogxK

U2 - 10.1016/j.neuroimage.2010.03.065

DO - 10.1016/j.neuroimage.2010.03.065

M3 - Article

C2 - 20362680

AN - SCOPUS:77953718613

SN - 1053-8119

VL - 52

SP - 562

EP - 570

JO - NeuroImage

JF - NeuroImage

IS - 2

ER -

Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this