IL-1β-induced MCP-1 expression and secretion of human dental pulp cells is related to TAK1, MEK/ERK, and PI3K/Akt signaling pathways

Mei Chi Chang, Yi Ling Tsai, Hsiao Hua Chang, Sheng Yang Lee, Ming Shu Lee, Chun Wei Chang, Chiu Po Chan, Chien Yang Yeh, Ru Hsiu Cheng, Jiiang Huei Jeng

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Objective Interleukin-1β (IL-1β) is an inflammatory molecule of the dental pulp. IL-1β stimulates cyclooxygenase-2 (COX-2) and prostaglandins production of pulp cells and affects the pulpal inflammation and repair. However, the effects of IL-1β on Monocyte Chemotactic Factor-1 (MCP-1) of dental pulp cells and its relation to transforming growth factor β-activated kinase-1 (TAK1), PI3K/Akt, and MEK/ERK signaling and COX activation are not fully clear. Design Human dental pulp cells were exposed to IL-1β with/without pretreatment and co-incubation by aspirin (a COX inhibitor), 5z-7-oxozeaenol (a TAK1 inhibitor), LY294002 (a PI3K/Akt inhibitor) or U0126 (a MEK/ERK inhibitor). Viable cell number was evaluated by MTT assay. MCP-1 mRNA expression was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). MCP-1 and COX-2 protein expression was studied by western blot. MCP-1 in the culture medium was measure by ELISA. Results IL-1β showed little cytotoxicity to pulp cells. It stimulated MCP-1 mRNA and protein expression and MCP-1 secretion. Aspirin, U0126, LY294002 and 5z-7-oxozeaenol attenuated the IL-1β-induced MCP-1 expression. In addition, 5z-7-oxozeaenol, LY294002, U0126 and aspirin prevented the IL-1β-induced MCP-1 secretion of pulp cells. Conclusion These results indicate that IL-1β may be involved in the pulpal inflammatory and healing processes by inducing MCP-1 expression and secretion. These events are related to differential activation of TAK1, PI3K/Akt and MEK/ERK 1/2 signaling and COX activation. These results are important for future pharmacologic intervention of pulpal inflammatory diseases.

Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalArchives of Oral Biology
Publication statusPublished - Jan 1 2016


  • Cyclooxygenase
  • Inflammation
  • Interleukin-1β
  • MCP-1
  • PI3K/Akt
  • Pulp
  • Signal transduction
  • TAK1

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry
  • Cell Biology


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