IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

Janet Rubin; Xian Fan; Jill Rahnert; Buer Sen; Chia Ling Hsieh; Tamara C. Murphy; Mark S. Nanes; Lindsay G. Horton; Wesley G. Beamer; Clifford J. Rosen

doi:10.1002/pros.20379

IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

Janet Rubin, Xian Fan, Jill Rahnert, Buer Sen, Chia Ling Hsieh, Tamara C. Murphy, Mark S. Nanes, Lindsay G. Horton, Wesley G. Beamer, Clifford J. Rosen

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

BACKGROUND. IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases. METHODS. Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT. RESULTS. CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response. CONCLUSIONS. IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.

Original language	English
Pages (from-to)	789-800
Number of pages	12
Journal	Prostate
Volume	66
Issue number	8
DOIs	https://doi.org/10.1002/pros.20379
Publication status	Published - Jun 1 2006
Externally published	Yes

Keywords

LNCaP
Osteoblast
Osteolysis
PC-3

ASJC Scopus subject areas

Oncology
Urology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/pros.20379

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title = "IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo",

abstract = "BACKGROUND. IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases. METHODS. Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT. RESULTS. CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response. CONCLUSIONS. IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.",

keywords = "LNCaP, Osteoblast, Osteolysis, PC-3",

author = "Janet Rubin and Xian Fan and Jill Rahnert and Buer Sen and Hsieh, {Chia Ling} and Murphy, {Tamara C.} and Nanes, {Mark S.} and Horton, {Lindsay G.} and Beamer, {Wesley G.} and Rosen, {Clifford J.}",

year = "2006",

month = jun,

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doi = "10.1002/pros.20379",

language = "English",

volume = "66",

pages = "789--800",

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TY - JOUR

T1 - IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

AU - Rubin, Janet

AU - Fan, Xian

AU - Rahnert, Jill

AU - Sen, Buer

AU - Hsieh, Chia Ling

AU - Murphy, Tamara C.

AU - Nanes, Mark S.

AU - Horton, Lindsay G.

AU - Beamer, Wesley G.

AU - Rosen, Clifford J.

PY - 2006/6/1

Y1 - 2006/6/1

N2 - BACKGROUND. IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases. METHODS. Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT. RESULTS. CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response. CONCLUSIONS. IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.

AB - BACKGROUND. IGF-I is an important growth and differentiative factor for osteoblasts and may have a role in defining prostate cancer risk and skeletal metastases. METHODS. Conditioned media (CM) from human prostate cancer (PC), C4-2 and C4-2B, which produce osteoblastic lesions, and PC-3, which causes osteolysis, was added to MC3T3-E1 bone cultures. SCID mice were injected intratibially with these engineered cells. Tumor bearing tibiae were analyzed by microCT and pQCT. RESULTS. CM from PC cells increased osteoblast proliferation and differentiation and was unaltered by the type of PC cell, IGF-I antibodies, or exogenous IGF-I and IGFBP2. Study of intratibial PC tumors in SCID mice showed that C4-2 cells grew slowly preserving bone structure, while PC-3 tumors caused rapid osteolysis. Overexpression of IGF-I did not change either tumor progression or skeletal response. CONCLUSIONS. IGF-I is neither necessary nor sufficient for the osteoblastic response to PC metastases.

KW - LNCaP

KW - Osteoblast

KW - Osteolysis

KW - PC-3

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IGF-I secretion by prostate carcinoma cells does not alter tumor-bone cell interactions in vitro or in vivo

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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