Ibuprofen augments pro-inflammatory cytokine release in a mouse model of Vibrio vulnificus infection

We evaluated the effects of ibuprofen on cytokine production and mortality in a mouse model of septic shock induced by Vibrio vulnificus, strain Chi Mei Vv05191. Ibuprofen (50 mg/kg) or saline (control) was given to female BALB/cByJ mice for three consecutive days before exposure to the pathogen. For cytokine production, serum and peritoneal fluid were assayed for IL-1β, IL-6, TNF-α, and MIP-2 by ELISA at 3, 6, and 9 hr after intraperitoneal infection of the organism. At 6 hr after infection, serum and peritoneal fluid levels of IL-6, TNF-α, and MIP-2 were significantly higher in the ibuprofen group. For mortality determination, 73 mice (37 ibuprofen, 36 control) were injected intramuscularly with V. vulnificus. Kaplan-Meier survival curves were analyzed. Survival was significantly decreased by ibuprofen only for the lowestinoculum(25 CFU)of V. vulnificus. Administration of ibuprofen before infectionmay augment the pathogenesis of V. vulnificus by stimulating cytokine production.