Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

Shankung Lin; Kou-Gi Shyu; Chun Chung Lee; Bao Wei Wang; Chih Chuan Chang; Ya Chen Liu; Fan Yen Huang; Hang Chang

doi:10.1016/S0006-291X(02)00924-5

Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

Shankung Lin, Kou-Gi Shyu, Chun Chung Lee, Bao Wei Wang, Chih Chuan Chang, Ya Chen Liu, Fan Yen Huang, Hang Chang

Graduate Institute of Injury Prevention and Control

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.

Original language	English
Pages (from-to)	710-715
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	296
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(02)00924-5
Publication status	Published - 2002

Keywords

Angiopoietin
Human umbilical vein endothelial cells
Hyperbaric oxygen
Nitric oxide synthase

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(02)00924-5

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title = "Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells",

abstract = "One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.",

keywords = "Angiopoietin, Human umbilical vein endothelial cells, Hyperbaric oxygen, Nitric oxide synthase",

author = "Shankung Lin and Kou-Gi Shyu and Lee, {Chun Chung} and Wang, {Bao Wei} and Chang, {Chih Chuan} and Liu, {Ya Chen} and Huang, {Fan Yen} and Hang Chang",

note = "Funding Information: This study was supported by the Research Committee of Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.",

year = "2002",

doi = "10.1016/S0006-291X(02)00924-5",

language = "English",

volume = "296",

pages = "710--715",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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T1 - Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

AU - Lin, Shankung

AU - Shyu, Kou-Gi

AU - Lee, Chun Chung

AU - Wang, Bao Wei

AU - Chang, Chih Chuan

AU - Liu, Ya Chen

AU - Huang, Fan Yen

AU - Chang, Hang

N1 - Funding Information: This study was supported by the Research Committee of Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan.

PY - 2002

Y1 - 2002

N2 - One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.

AB - One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells exposed to HBO. Western blot analyses showed that HBO enhanced the expression of angiopoietin-2 (Ang2) with no effect on the expression of Tie2, angiopoietin-1, and VEGF. The induction of Ang2 was further confirmed by immunohistochemistry, quantitative PCR, and Northern blot analyses. Inhibition of endothelial nitric oxide synthase blocked the HBO-induced Ang2 expression, but failed to block hypoxia-induced Ang2 expression. These data indicated that HBO-induced Ang2 expression may be through transcriptional stimulation, and requires the nitric oxide signaling pathway, which may play an important role in HBO-induced angiogenesis.

KW - Angiopoietin

KW - Human umbilical vein endothelial cells

KW - Hyperbaric oxygen

KW - Nitric oxide synthase

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U2 - 10.1016/S0006-291X(02)00924-5

DO - 10.1016/S0006-291X(02)00924-5

M3 - Article

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AN - SCOPUS:0036382833

SN - 0006-291X

VL - 296

SP - 710

EP - 715

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

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