Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

Yueh Jen Chen; Ren Guei Wu; Peng Chun Chen; Yu Nan Lin; Huei Wen Hsiu; Ching Chu Yen; Chia-Woei Wang; Li-Chern Pan; Fan Gang Tseng

doi:10.1109/NEMS.2016.7758215

Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

Yueh Jen Chen, Ren Guei Wu, Peng Chun Chen, Yu Nan Lin, Huei Wen Hsiu, Ching Chu Yen, Chia-Woei Wang, Li-Chern Pan, Fan Gang Tseng

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

Original language	English
Title of host publication	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	130-133
Number of pages	4
ISBN (Electronic)	9781509019472
DOIs	https://doi.org/10.1109/NEMS.2016.7758215
Publication status	Published - Nov 28 2016
Event	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan Duration: Apr 17 2016 → Apr 20 2016

Publication series

Name	2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016

Other

Other	11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
Country/Territory	Japan
City	Sendai
Period	4/17/16 → 4/20/16

Keywords

CASA
Chip
PDMS
Sperm

Access to Document

10.1109/NEMS.2016.7758215

Cite this

Chen, Y. J., Wu, R. G., Chen, P. C., Lin, Y. N., Hsiu, H. W., Yen, C. C., Wang, C.-W., Pan, L.-C., & Tseng, F. G. (2016). Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 130-133). Article 7758215 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758215

Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. / Chen, Yueh Jen; Wu, Ren Guei; Chen, Peng Chun et al.
2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 130-133 7758215 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Chen, YJ, Wu, RG, Chen, PC, Lin, YN, Hsiu, HW, Yen, CC, Wang, C-W, Pan, L-C & Tseng, FG 2016, Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. in 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758215, 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Institute of Electrical and Electronics Engineers Inc., pp. 130-133, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 4/17/16. https://doi.org/10.1109/NEMS.2016.7758215

Chen YJ, Wu RG, Chen PC, Lin YN, Hsiu HW, Yen CC et al. Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 130-133. 7758215. (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016). doi: 10.1109/NEMS.2016.7758215

Chen, Yueh Jen ; Wu, Ren Guei ; Chen, Peng Chun et al. / Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 130-133 (2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016).

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abstract = "This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).",

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AU - Hsiu, Huei Wen

AU - Yen, Ching Chu

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AU - Pan, Li-Chern

AU - Tseng, Fan Gang

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N2 - This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

AB - This paper proposes a selection and extraction strategy to acquire high quality sperms from a biomimetic microfluidic device capable of generating a dual gradient flow field for motile sperm sorting. The sorter consists of a straight flow zone, a rapidly expansion sector for sperm sorting by gradient up-stream flow, and a dumbbell channel for dead sperms separation at the outlet. A collateral runner is set at the front area of the expansion sector, but mostly kept in dry state without affecting the sorting process. At the end of sorting, a pressure was applied from both the inlet and outlet to flush the high quality sperms into the collateral runner for collection. Controllable numbers, ranging from 1 to 15000, of sperms can be collected with a motility (>60μm) ratio higher than 60%, suitable for in-vitro fertilization (IVF).

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KW - Chip

KW - PDMS

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Hydraulic extraction of high quality sperms from a dual gradient sperm sorter for in-vitro fertilizatio

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