Abstract
The carbohydrate polymer, hyaluronan, is a major component of the extracellular matrix in animal tissues. Exogenous hyaluronan has been used to treat osteoarthritis (OA), a degenerative joint disease involving inflammatory changes. The underlying mechanisms of hyaluronan in OA are not fully understood. Pro-inflammatory interleukin (IL)-1β downregulates peroxisome proliferator-activated receptor gamma (PPARγ), and increases expression of matrix metalloproteinases (MMPs) which are responsible for the degeneration of articular cartilage. The effects of low- and high-molecular-weight hyaluronan (oligo-HA and HMW-HA) on the inflammatory genes were determined in human SW-1353 chondrosarcoma cells. HMW-HA antagonized the effects of IL-1β by increasing PPARγ and decreasing cyclooxygenase (COX)-2, MMP-1, and MMP-13 levels. It promoted Akt, but suppressed mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NFκB) signaling, indicating anti-inflammatory effects. In contrast, the cells had overall opposite responses to oligo-HA. In conclusion, HMW-HA and oligo-HA exerted differential inflammatory responses via PPARγ in IL-1β-treated chondrosarcoma cells.
Original language | English |
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Pages (from-to) | 1168-1175 |
Number of pages | 8 |
Journal | Carbohydrate Polymers |
Volume | 90 |
Issue number | 2 |
DOIs | |
Publication status | Published - Oct 1 2012 |
Keywords
- Cyclooxygenase-2
- Hyaluronan
- Matrix metalloproteinase
- Osteoarthritis
- Peroxisome proliferator-activated receptor
ASJC Scopus subject areas
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry