TY - JOUR
T1 - Human urinary bladder cancer T24 cells are susceptible to the Antrodia camphorata extracts
AU - Peng, Chiung Chi
AU - Chen, Kuan Chou
AU - Peng, Robert Y.
AU - Su, Ching-Hua
AU - Hsieh-Li, Hsiu Mei
PY - 2006/11/8
Y1 - 2006/11/8
N2 - Bladder cancer has been cited to result from the neoplastic lesion with environmental and/or occupational factors identified as causatives. Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Most of the bladder cancer patients die from the invasive, metastatic TCC that has turned out to be resistant to chemotherapy. T24 cells, a cell line established from a human urinary bladder cancer patient, are high-grade and invasive TCC. T24 cells were found very susceptible to ACCE at concentration of 50 μg/mL. MTT assay showed that the cell growth and proliferation were inhibited to 50% of the control when treated with ACCE for 72 h, at which the cell proliferation suppressing rate revealed -4.4×103 cells/μg per day. Comparing the expressions of the cell cycle biomarkers Cdc2 and Cyclin B1 by the western blot analysis, a phase G2M arrest was confirmed. Both the wound scratch assay and the transwell motility assay indicated that ACCE was very effective anti-metastatic against T24 cells. Furthermore, the active form of matrix metalloproteinase-9 (MMP-9) was also found totally suppressed as revealed by zymography at 72 h post-incubation with ACCE, while the light and electron microscopic images have apparently revealed cell membrane damages on T24 cells when treated with ACCE (50 μg/mL). Moreover, both the wound scratch and the transwell assays have demonstrated the migration capability of T24 cells has been significantly retarded to 1.5-fold at same dosage of ACCE used. In conclusion, ACCE is a good anti-cancer agent, being effective in inducing phase G2M arrest, acting as an anti-proliferative, and an anti-metastatic agent against bladder cancer cell T24 cells.
AB - Bladder cancer has been cited to result from the neoplastic lesion with environmental and/or occupational factors identified as causatives. Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Most of the bladder cancer patients die from the invasive, metastatic TCC that has turned out to be resistant to chemotherapy. T24 cells, a cell line established from a human urinary bladder cancer patient, are high-grade and invasive TCC. T24 cells were found very susceptible to ACCE at concentration of 50 μg/mL. MTT assay showed that the cell growth and proliferation were inhibited to 50% of the control when treated with ACCE for 72 h, at which the cell proliferation suppressing rate revealed -4.4×103 cells/μg per day. Comparing the expressions of the cell cycle biomarkers Cdc2 and Cyclin B1 by the western blot analysis, a phase G2M arrest was confirmed. Both the wound scratch assay and the transwell motility assay indicated that ACCE was very effective anti-metastatic against T24 cells. Furthermore, the active form of matrix metalloproteinase-9 (MMP-9) was also found totally suppressed as revealed by zymography at 72 h post-incubation with ACCE, while the light and electron microscopic images have apparently revealed cell membrane damages on T24 cells when treated with ACCE (50 μg/mL). Moreover, both the wound scratch and the transwell assays have demonstrated the migration capability of T24 cells has been significantly retarded to 1.5-fold at same dosage of ACCE used. In conclusion, ACCE is a good anti-cancer agent, being effective in inducing phase G2M arrest, acting as an anti-proliferative, and an anti-metastatic agent against bladder cancer cell T24 cells.
KW - Antrodia camphorata
KW - Matrix metalloproteinase (MMP)
KW - Migration
KW - Transitional cell carcinoma (TCC)
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UR - http://www.scopus.com/inward/citedby.url?scp=33749241470&partnerID=8YFLogxK
U2 - 10.1016/j.canlet.2005.11.021
DO - 10.1016/j.canlet.2005.11.021
M3 - Article
C2 - 16455193
AN - SCOPUS:33749241470
SN - 0304-3835
VL - 243
SP - 109
EP - 119
JO - Cancer Letters
JF - Cancer Letters
IS - 1
ER -