Human umbilical cord mesenchymal stem cells in combination with hyaluronic acid ameliorate the progression of knee osteoarthritis

Jia Lin Wu, Pei Chun Wong, Chung Wei Ho, Chien Han Chen, Kuan Ya Liao, Ronald Lovel, Tang Bo Chung Wu, Wen Ying Chang, Yan Zhang Lee, Willie Lin

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The aim of this study is to evaluate the feasibility and usefulness of the human umbilical cord mesenchymal stem cells (hUC-MSCs) and hyaluronan acid (HA) combination to attenuate osteoarthritis progression in the knee while simultaneously providing some insights on the mitigation mechanism. In vitro, the effect of hUC-MSCs with HA treatment on chondrocyte cell viability and the cytokine profile were analyzed. Additionally, the antioxidation capability of hUC-MSCs-CM (conditioned medium) with HA towards H2O2-induced chondrocyte cell damage was evaluated. The HA addition increased the hUC-MSC antioxidation capability and cytokine secretion, such as Dickkopf-related protein 1 (DKK-1) and hepatocyte growth factor (HGF), while no adverse effect on the cell viability was observed. In vivo, the intra-articular injection of hUC-MSCs with HA to a monoiodoacetate (MIA)-induced knee osteoarthritis (KOA) rat model was performed and investigated. Attenuation of the KOA progression in the MIA-damaged rat model was seen best in hUC-MSCs with a HA combination compared to the vehicle control or each individual element. Combining hUC-MSCs and HA resulted in a synergistic effect, such as increasing the cell therapeutic capability while incurring no observable adverse effects. Therefore, this combinatorial therapy is feasible and has promising potential to ameliorate KOA progression.

Original languageEnglish
Article number6650
JournalApplied Sciences (Switzerland)
Issue number14
Publication statusPublished - Jul 2021


  • Antioxidation
  • DKK-1
  • Hyaluronan acid
  • Mesenchymal stem cell
  • Osteoarthritis

ASJC Scopus subject areas

  • General Materials Science
  • Instrumentation
  • General Engineering
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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