Human papillomavirus genotype in cervical intraepithelial neoplasia grades 2 and 3 of Taiwanese women

Angel Chao, Mei Shan Jao, Chu Chun Huang, Huei Jean Huang, Hui Hsin Cheng, Jung Erh Yang, Swei Hsueh, Tse Ching Chen, Cheng Tao Lin, Chang Jui Fu, Hung Hsueh Chou, Chyong Huey Lai, J. Timothy Qiu

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16 Citations (Scopus)


We aimed to assess the distribution of human papillomavirus (HPV) genotypes in high-grade cervical lesions in Taiwan. The study included 1,086 paraffin-embedded, formaldehyde-fixed cervical intraepithelial neoplasia (CIN) 2/3 specimens. HPV genotyping was performed using polymerase chain reaction (PCR)-based methods. Multiple HPV types were validated by E6 type-specific PCR, direct sequencing and/or real-time PCR. HPV DNA was detected in 995 (91.6%) specimens, and multiple HPV types were identified in 192 (19.3%) samples. The leading HPV types were HPV16 (24%), HPV52 (20%), HPV58 (20%), HPV33 (13%), HPV31 (8%) and HPV18 (4.6%). Although the leading six types consisted of 87.6%, HPV16 or 18 comprised only 30.9%. The prevalence of different HPV types showed a significant association with age. In women older than 50 yr, HPV16 and 18 comprised 21.3% (83/389), while HPV52, 58 and 33 represented 55.5% (216/389). In women aged less than 50 yr, HPV16 and 18 comprised 32.1% (224/697, p < 0.0001), while HPV 52, 58 and 33 represented 47.9% (334/697, p = 0.02). The distribution of HPV genotypes was compared with previously reported findings for Taiwanese women with cervical cancer (CC). The overall HPV16 positivity rate was significantly higher in CC than in CIN 2/3 (odds ratio: 2.14, 95% CI: 1.91-2.40). In addition, HPV18, 39 and 45 were significantly overrepresented in CC, whereas HPV52, 58, 33, 31, 35, 51 and 53 were underrepresented. We concluded that an effective vaccine against the most common HPV types could prevent a significant proportion of cervical cancer cases that occur in Taiwan.

Original languageEnglish
Pages (from-to)653-659
Number of pages7
JournalInternational Journal of Cancer
Issue number3
Publication statusPublished - Feb 1 2011


  • cervical intraepithelial neoplasia
  • conization
  • genotype
  • HPV

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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