Human chromosomal centromere (AATGG)n sequence forms stable structures with unusual base pairs

T. N. Jaishree; Andrew H.J. Wang

doi:10.1016/0014-5793(94)00516-8

Human chromosomal centromere (AATGG)_n sequence forms stable structures with unusual base pairs

T. N. Jaishree
, Andrew H.J. Wang

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG)_n·(CCAAT)_n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG)_n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.

Original language	English
Pages (from-to)	99-103
Number of pages	5
Journal	FEBS Letters
Volume	347
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(94)00516-8
Publication status	Published - Jun 20 1994
Externally published	Yes

Keywords

Centromere sequence
DNA structure
NMR
Unusual base pairs

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(94)00516-8

Cite this

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title = "Human chromosomal centromere (AATGG)n sequence forms stable structures with unusual base pairs",

abstract = "Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG)n·(CCAAT)n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG)n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.",

keywords = "Centromere sequence, DNA structure, NMR, Unusual base pairs",

author = "Jaishree, \{T. N.\} and Wang, \{Andrew H.J.\}",

note = "Funding Information: Acknowledgements: This work was supported by NIH Grants GM-41612 and CA-52506 to A.H.-J.W. The Varian VXR500 spectrometer was supported by NIH shared Instrumentation Grant 1S10RR06243.",

year = "1994",

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doi = "10.1016/0014-5793(94)00516-8",

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AU - Jaishree, T. N.

AU - Wang, Andrew H.J.

N1 - Funding Information: Acknowledgements: This work was supported by NIH Grants GM-41612 and CA-52506 to A.H.-J.W. The Varian VXR500 spectrometer was supported by NIH shared Instrumentation Grant 1S10RR06243.

PY - 1994/6/20

Y1 - 1994/6/20

N2 - Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG)n·(CCAAT)n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG)n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.

AB - Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG)n·(CCAAT)n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG)n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.

KW - Centromere sequence

KW - DNA structure

KW - NMR

KW - Unusual base pairs

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