TY - JOUR
T1 - Human adipose-derived stem cell secreted extracellular matrix incorporated into electrospun poly(lactic-co-glycolic acid) nanofibrous dressing for enhancing wound healing
AU - Tang, Kao Chun
AU - Yang, Kai Chiang
AU - Lin, Che Wei
AU - Chen, Yi Kai
AU - Lu, Ting Yu
AU - Chen, Hsien Yeh
AU - Cheng, Nai Chen
AU - Yu, Jiashing
N1 - Funding Information:
Funding: This research was funded by Ministry of Science of Technology, and Taiwan and Higher Education Sprout Project (108L7826), Ministry of Education, Taiwan.
Funding Information:
Acknowledgments: This work was supported by the Ministry of Science and Technology, Taiwan. The authors are grateful to the Department of Surgery of National Taiwan University Hospital for providing human adipose-derived stem cells. We also appreciate the technical assistance provided by the students of the department of Chemical Engineering of National Taiwan University.
Funding Information:
This research was funded by Ministry of Science of Technology, and Taiwan and Higher Education Sprout Project (108L7826), Ministry of Education, Taiwan. This work was supported by the Ministry of Science and Technology, Taiwan. The authors are grateful to the Department of Surgery of National Taiwan University Hospital for providing human adipose-derived stem cells. We also appreciate the technical assistance provided by the students of the department of Chemical Engineering of National Taiwan University.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days' cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing.
AB - Wound dressing, which prevents dehydration and provides a physical barrier against infection to wound beds, can improve wound healing. The interactions between extracellular matrix (ECM) and growth factors is critical to the healing process. Electrospun nanofibers are promising templates for wound dressings due to the structure similarity to ECM of skin. Otherwise, the ECM secreted by human adipose-derived stem cells (hASCs) is rich in growth factors known to enhance wound healing. Accordingly, we propose that the PLGA nanofibrous template incorporated with hASCs-secreted ECM may enhance wound healing. In this study, PLGA nanofibrous matrixes with an aligned or a random structure were prepared by electrospinning. Human ASCs cultured on the aligned matrix had a better viability and produced a larger amount of ECM relative to that of random one. After 7 days' cultivation, the hASCs on aligned PLGA substrates underwent decellularization to fabricate cECM/PLGA dressings. By using immunohistochemical staining against F-actin and cell nucleus, the removal of cellular components was verified. However, the type I collagen and laminin were well preserved on the cECM/PLGA nanofibrous matrixes. In addition, this substrate was hydrophilic, with appropriate mechanical strength to act as a wound dressing. The L929 fibroblasts had good activity, survival and proliferation on the cECM/PLGA meshes. In addition, the cECM/PLGA nanofibrous dressings improved the wound healing of surgically created full-thickness skin excision in a mouse model. This hASCs-secreted ECM incorporated into electrospun PLGA nanofibrous could be a promising dressing for enhancing wound healing.
KW - Electrospinning
KW - Extracellular matrix
KW - Human adipose-derived stem cells
KW - Wound healing
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U2 - 10.3390/polym11101609
DO - 10.3390/polym11101609
M3 - Article
AN - SCOPUS:85073379626
SN - 2073-4360
VL - 11
JO - Polymers
JF - Polymers
IS - 10
M1 - 1609
ER -