Abstract
We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS structures with vertical vias connecting fluid in different layers. An array of high N.A. microball lens is also embedded in the structure to achieve parallel and high sensitivity fluorescence detection across these 32 channels.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1909-1911 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806452 |
| Publication status | Published - 2012 |
| Externally published | Yes |
| Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: Oct 28 2012 → Nov 1 2012 |
Conference
| Conference | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
|---|---|
| Country/Territory | Japan |
| City | Okinawa |
| Period | 10/28/12 → 11/1/12 |
Keywords
- 3D microfluidics
- FACS
- Flow cytometry
- Microlens
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering