High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

Yu Jui Fan; Yen Ling Weng; Horn Jiunn Sheen

doi:10.1109/NEMS.2017.8017057

High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

Yu Jui Fan, Yen Ling Weng, Horn Jiunn Sheen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

Original language	English
Title of host publication	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	426-429
Number of pages	4
ISBN (Electronic)	9781509030590
DOIs	https://doi.org/10.1109/NEMS.2017.8017057
Publication status	Published - Aug 25 2017
Event	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States Duration: Apr 9 2017 → Apr 12 2017

Publication series

Name	2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference	12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Country/Territory	United States
City	Los Angeles
Period	4/9/17 → 4/12/17

Keywords

BioMEMS
flow cytometry
microfluidics

ASJC Scopus subject areas

Electrical and Electronic Engineering
Mechanical Engineering
Electronic, Optical and Magnetic Materials
Instrumentation

Access to Document

10.1109/NEMS.2017.8017057

Cite this

Fan, Y. J., Weng, Y. L., & Sheen, H. J. (2017). High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 (pp. 426-429). Article 8017057 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2017.8017057

High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. / Fan, Yu Jui; Weng, Yen Ling; Sheen, Horn Jiunn.
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 426-429 8017057 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fan, YJ, Weng, YL & Sheen, HJ 2017, High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. in 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017., 8017057, 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Institute of Electrical and Electronics Engineers Inc., pp. 426-429, 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017, Los Angeles, United States, 4/9/17. https://doi.org/10.1109/NEMS.2017.8017057

Fan YJ, Weng YL, Sheen HJ. High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. In 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 426-429. 8017057. (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017). doi: 10.1109/NEMS.2017.8017057

Fan, Yu Jui ; Weng, Yen Ling ; Sheen, Horn Jiunn. / High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device. 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 426-429 (2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017).

@inproceedings{6190214bab0d441fb0f38608040274f0,

title = "High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device",

abstract = "We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.",

keywords = "BioMEMS, flow cytometry, microfluidics",

author = "Fan, {Yu Jui} and Weng, {Yen Ling} and Sheen, {Horn Jiunn}",

note = "Publisher Copyright: {\textcopyright} 2017 IEEE.; 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 ; Conference date: 09-04-2017 Through 12-04-2017",

year = "2017",

month = aug,

day = "25",

doi = "10.1109/NEMS.2017.8017057",

language = "English",

series = "2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "426--429",

booktitle = "2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017",

}

TY - GEN

T1 - High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

AU - Fan, Yu Jui

AU - Weng, Yen Ling

AU - Sheen, Horn Jiunn

PY - 2017/8/25

Y1 - 2017/8/25

N2 - We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

AB - We report an ultrahigh throughput microflow cytometer which has 32 detection flow channels with 3D flow focusing for each channel. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels. There are, 64 channels for sheath flow used for parallel lateral focusing and 32 sounding flows for parallel sounding focusing. All microchannels use only three inlets. Results show that a 95% yield rate by standard 10 μm bead detection has been achieved, and a throughput of 1,400,000 cell/sec has been accomplished on a single device.

KW - BioMEMS

KW - flow cytometry

KW - microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85030863778&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030863778&partnerID=8YFLogxK

U2 - 10.1109/NEMS.2017.8017057

DO - 10.1109/NEMS.2017.8017057

M3 - Conference contribution

AN - SCOPUS:85030863778

T3 - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

SP - 426

EP - 429

BT - 2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Y2 - 9 April 2017 through 12 April 2017

ER -

High throughput and parallel flow cytometer using microball lens integrated 3D microfluidic device

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this