Abstract
An internal ribosome entry site (IRES) has been used to facilitate the expression of more than one protein in a single transcript. In this study, we examined the translational activities of several IRES elements derived from different RNA viruses. The protein expression of encephalomyocarditis virus (EMCV) IRES is similar to that of hepatitis C virus (HCV) IRES in mammalian cells. Notably, the protein expression of enterovirus 71 (EV71) IRES was 23-fold higher than the efficiency of EMCV IRES following normalization of mRNA transcriptional level. Thus, expression of the secreted alkaline phosphatase (SEAP) reporter protein in mammalian cells may be controlled at desirable levels by using appropriate IRES in the expression vector.
| Original language | English |
|---|---|
| Pages (from-to) | 656-662 |
| Number of pages | 7 |
| Journal | Biotechnology and Bioengineering |
| Volume | 90 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Jun 5 2005 |
| Externally published | Yes |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
-
SDG 3 Good Health and Well-being
Keywords
- EMCV
- EV71
- HCV
- IRES
- Polycistronic
- Protein expression
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Fingerprint
Dive into the research topics of 'High-efficiency protein expression mediated by enterovirus 71 internal ribosome entry site'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS