Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

Yi Kai Chen; Chih Hsiang Leng; Heidi Olivares; Ming Hui Lee; Yuan Chih Chang; Wen Mei Kung; Shih Chieh Ti; Yu Hui Lo; Andrew H.J. Wang; Chia Seng Chang; Douglas K. Bishop; Yi Ping Hsueh; Ting Fang Wang

doi:10.1073/pnas.0404195101

Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

Yi Kai Chen, Chih Hsiang Leng, Heidi Olivares, Ming Hui Lee, Yuan Chih Chang, Wen Mei Kung, Shih Chieh Ti, Yu Hui Lo, Andrew H.J. Wang, Chia Seng Chang, Douglas K. Bishop, Yi Ping Hsueh, Ting Fang Wang

Research output: Contribution to journal › Article › peer-review

94 Citations (Scopus)

Abstract

Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosis-specific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

Original language	English
Pages (from-to)	10572-10577
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	101
Issue number	29
DOIs	https://doi.org/10.1073/pnas.0404195101
Publication status	Published - Jul 20 2004
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.0404195101

Cite this

Chen, Y. K., Leng, C. H., Olivares, H., Lee, M. H., Chang, Y. C., Kung, W. M., Ti, S. C., Lo, Y. H., Wang, A. H. J., Chang, C. S., Bishop, D. K., Hsueh, Y. P., & Wang, T. F. (2004). Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation. Proceedings of the National Academy of Sciences of the United States of America, 101(29), 10572-10577. https://doi.org/10.1073/pnas.0404195101

Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation. / Chen, Yi Kai; Leng, Chih Hsiang; Olivares, Heidi et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 29, 20.07.2004, p. 10572-10577.

Research output: Contribution to journal › Article › peer-review

Chen, YK, Leng, CH, Olivares, H, Lee, MH, Chang, YC, Kung, WM, Ti, SC, Lo, YH, Wang, AHJ, Chang, CS, Bishop, DK, Hsueh, YP & Wang, TF 2004, 'Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 29, pp. 10572-10577. https://doi.org/10.1073/pnas.0404195101

@article{38c46547a30648aaadbec7f11b4b77e4,

title = "Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation",

abstract = "Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosis-specific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.",

author = "Chen, {Yi Kai} and Leng, {Chih Hsiang} and Heidi Olivares and Lee, {Ming Hui} and Chang, {Yuan Chih} and Kung, {Wen Mei} and Ti, {Shih Chieh} and Lo, {Yu Hui} and Wang, {Andrew H.J.} and Chang, {Chia Seng} and Bishop, {Douglas K.} and Hsueh, {Yi Ping} and Wang, {Ting Fang}",

year = "2004",

month = jul,

day = "20",

doi = "10.1073/pnas.0404195101",

language = "English",

volume = "101",

pages = "10572--10577",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "29",

}

TY - JOUR

T1 - Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

AU - Chen, Yi Kai

AU - Leng, Chih Hsiang

AU - Olivares, Heidi

AU - Lee, Ming Hui

AU - Chang, Yuan Chih

AU - Kung, Wen Mei

AU - Ti, Shih Chieh

AU - Lo, Yu Hui

AU - Wang, Andrew H.J.

AU - Chang, Chia Seng

AU - Bishop, Douglas K.

AU - Hsueh, Yi Ping

AU - Wang, Ting Fang

PY - 2004/7/20

Y1 - 2004/7/20

N2 - Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosis-specific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

AB - Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosis-specific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination.

UR - http://www.scopus.com/inward/record.url?scp=3242690413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242690413&partnerID=8YFLogxK

U2 - 10.1073/pnas.0404195101

DO - 10.1073/pnas.0404195101

M3 - Article

C2 - 15249670

AN - SCOPUS:3242690413

SN - 0027-8424

VL - 101

SP - 10572

EP - 10577

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 29

ER -

Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this