Helicobacter pylori attenuates lipopolysaccharide-induced nitric oxide production by murine macrophages

Dah Yuu Lu, Chin Hsin Tang, Chia Hsian Chang, Ming Chei Maa, Shih Hua Fang, Yuan Man Hsu, Yu Hsin Lin, Chun Jung Lin, Wan Chi Lee, Hwai Jeng Lin, Che Hsin Lee, Chih Ho Lai

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Intragastric growth of Helicobacter pylori and non-Helicobacter microorganisms is thought to be associated with elevated levels of pro-inflammatory cytokines and the production of NO these effects can lead to chronic inflammation. Microorganisms can activate the expression of iNOS and the production of NO by macrophages through stimulation with bacterial LPS. Helicobacter pylori can evade these vigorous immune responses, but the underlying mechanism remains unknown. In this study, we used a murine model of macrophage infection to demonstrate that H. pylori inhibits LPS-induced expression of iNOS and production of NO by macrophages. Suppression of LPS-induced NO production by macrophages led to elevated survival of H. pylori in a trans-well system. This effect was abrogated in macrophages from iNOS-/- mice. Analysis of iNOS mRNA and protein levels revealed that H. pylori inhibits iNOS expression at both transcriptional and post-transcriptional levels, and that these effects occurred with live bacteria. Furthermore, the effect of H. pylori involved down-regulation of the mitogen-activated protein kinase pathway and the translocation of active NF-κB into the nucleus. Taken together, our results reveal a new mechanism by which H. pylori modulates the innate immune responses of the host and maintains a persistent infection within the stomach.

Original languageEnglish
Pages (from-to)406-417
Number of pages12
JournalInnate Immunity
Issue number3
Publication statusPublished - Jun 2012
Externally publishedYes


  • Helicobacter pylori
  • Lipopolysaccharide
  • NF-κB
  • macrophage
  • nitric oxide

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases


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