HeLa toposiomerase I

Leroy Fliu

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Similar to the prokaryotic systems, there are two types of DNA topoisomerases in animal cells. Both type I and type II DNA topoisomerases are purified to homogeneity from HeLa cells. The type I DNA topoisomerase from HeLa cells (HeLa topoisomerase I), similar to all other eukaryotic type I DNA topoisomerases, introduces transient single-stranded breaks on duplex DNA, resulting in the relaxation of a superhelical DNA or other types of topological rearrangements of DNA. HeLa topoisomerase I can also spontaneously break down single-stranded DNA, generating active enzyme-linked fragments of single-stranded DNA. The exact biological functions of animal topoisomerase I are still unknown. Processes such as DNA sequence rearrangements, DNA replication, RNA transcription, and genetic recombination are suggested for such an activity. This chapter explains the assay method for HeLa topoisomerase I.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalMethods in Enzymology
Issue numberC
Publication statusPublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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