HeLa toposiomerase I

Leroy Fliu

doi:10.1016/0076-6879(83)00049-X

HeLa toposiomerase I

Leroy Fliu

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Similar to the prokaryotic systems, there are two types of DNA topoisomerases in animal cells. Both type I and type II DNA topoisomerases are purified to homogeneity from HeLa cells. The type I DNA topoisomerase from HeLa cells (HeLa topoisomerase I), similar to all other eukaryotic type I DNA topoisomerases, introduces transient single-stranded breaks on duplex DNA, resulting in the relaxation of a superhelical DNA or other types of topological rearrangements of DNA. HeLa topoisomerase I can also spontaneously break down single-stranded DNA, generating active enzyme-linked fragments of single-stranded DNA. The exact biological functions of animal topoisomerase I are still unknown. Processes such as DNA sequence rearrangements, DNA replication, RNA transcription, and genetic recombination are suggested for such an activity. This chapter explains the assay method for HeLa topoisomerase I.

Original language	English
Pages (from-to)	133-137
Number of pages	5
Journal	Methods in Enzymology
Volume	100
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(83)00049-X
Publication status	Published - Jan 1 1983
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0076-6879(83)00049-X

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HeLa toposiomerase I

Abstract

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