HDAC1,2 Knock-Out and HDACi Induced Cell Apoptosis in Imatinib-Resistant K562 Cells

Shu Huey Chen, Jyh Ming Chow, Yao Yu Hsieh, Chun Yu Lin, Kai Wen Hsu, Wen Shyang Hsieh, Wei Ming Chi, Beished M. Shabangu, Chia Hwa Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Since imatinib (Glivec or Gleevec) has been used to target the BCR-ABL fusion protein, chronic myeloid leukemia (CML) has become a manageable chronic disease with long-term survival. However, 15%-20% of CML patients ultimately develop resistance to imatinib and then progress to an accelerated phase and eventually to a blast crisis, limiting treatment options and resulting in a poor survival rate. Thus, we investigated whether histone deacetylase inhibitors (HDACis) could be used as a potential anticancer therapy for imatinib-resistant CML (IR-CML) patients. By applying a noninvasive apoptosis detection sensor (NIADS), we found that panobinostat significantly enhanced cell apoptosis in K562 cells. A further investigation showed that panobinostat induced apoptosis in both K562 and imatinib-resistant K562 (IR-K562) cells mainly via H3 and H4 histone acetylation, whereas panobinostat targeted cancer stem cells (CSCs) in IR-K562 cells. Using CRISPR/Cas9 genomic editing, we found that HDAC1 and HDAC2 knockout cells significantly induced cell apoptosis, indicating that the regulation of HDAC1 and HDAC2 is extremely important in maintaining K562 cell survival. All information in this study indicates that regulating HDAC activity provides therapeutic benefits against CML and IR-CML in the clinic.

Original languageEnglish
Article number2271
JournalInternational Journal of Molecular Sciences
Issue number9
Publication statusPublished - May 1 2019


  • CML
  • CRISPR/Cas9
  • histone deacetylase inhibitor
  • imatinib
  • imatinib-resistant
  • Acetylation/drug effects
  • Histone Deacetylase 2/genetics
  • Humans
  • Apoptosis/drug effects
  • Drug Resistance, Neoplasm/drug effects
  • Neoplastic Stem Cells/drug effects
  • Gene Knockout Techniques
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy
  • CRISPR-Cas Systems/genetics
  • Imatinib Mesylate/adverse effects
  • K562 Cells
  • Histone Deacetylase Inhibitors/pharmacology
  • Fusion Proteins, bcr-abl/antagonists & inhibitors
  • Histone Deacetylase 1/genetics
  • Panobinostat/pharmacology

ASJC Scopus subject areas

  • Molecular Biology
  • Spectroscopy
  • Catalysis
  • Inorganic Chemistry
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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