Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Tsai Mu Cheng; Simon J T Mao; Shiau Ting Lai; Chia Ching Chang; Ming Chi Yang; Nai Chi Chen; Shiu Ching Chou; Ju Pin Pan

doi:10.3109/10715762.2010.532492

Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Tsai Mu Cheng, Simon J T Mao, Shiau Ting Lai, Chia Ching Chang, Ming Chi Yang, Nai Chi Chen, Shiu Ching Chou, Ju Pin Pan

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H ₂O ₂ generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H ₂O ₂ decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H ₂O ₂ production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.

Original language	English
Pages (from-to)	303-316
Number of pages	14
Journal	Free Radical Research
Volume	45
Issue number	3
DOIs	https://doi.org/10.3109/10715762.2010.532492
Publication status	Published - Mar 2011
Externally published	Yes

Keywords

Hemoglobin
ICAM-1
LDL peroxidation
VCAM-1
cell adhesion
peroxidase
probucol

ASJC Scopus subject areas

Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/10715762.2010.532492

Cite this

@article{f6845d41ffa4456b9d4a9e3331d43f25,

title = "Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids",

abstract = "Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.",

keywords = "Hemoglobin, ICAM-1, LDL peroxidation, VCAM-1, cell adhesion, peroxidase, probucol",

author = "Cheng, {Tsai Mu} and Mao, {Simon J T} and Lai, {Shiau Ting} and Chang, {Chia Ching} and Yang, {Ming Chi} and Chen, {Nai Chi} and Chou, {Shiu Ching} and Pan, {Ju Pin}",

note = "Funding Information: This work was supported by grants from the National Health Research Institute (NHRI-EX92-9229SI [S.J.T.M.]) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH90-2-60A [J.P.P]).",

year = "2011",

month = mar,

doi = "10.3109/10715762.2010.532492",

language = "English",

volume = "45",

pages = "303--316",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "3",

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TY - JOUR

T1 - Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

AU - Cheng, Tsai Mu

AU - Mao, Simon J T

AU - Lai, Shiau Ting

AU - Chang, Chia Ching

AU - Yang, Ming Chi

AU - Chen, Nai Chi

AU - Chou, Shiu Ching

AU - Pan, Ju Pin

N1 - Funding Information: This work was supported by grants from the National Health Research Institute (NHRI-EX92-9229SI [S.J.T.M.]) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH90-2-60A [J.P.P]).

PY - 2011/3

Y1 - 2011/3

N2 - Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.

AB - Patients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression.

KW - Hemoglobin

KW - ICAM-1

KW - LDL peroxidation

KW - VCAM-1

KW - cell adhesion

KW - peroxidase

KW - probucol

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ER -

Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H2O2 generation from polyunsaturated fatty acids

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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