Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering

Peng Yuan Wang; Tsung Han Wu; Wei Bor Tsai; Wei Hsuan Kuo; Meng Jiy Wang

doi:10.1016/j.colsurfb.2013.04.016

Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering

Peng Yuan Wang, Tsung Han Wu, Wei Bor Tsai, Wei Hsuan Kuo, Meng Jiy Wang

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Alignment of myocytes or myotubes is critical for skeletal muscle tissue engineering. In this study, grooved PLGA films (800. nm in width of ridge/groove and 600. nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for skeletal muscle tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for skeletal muscle tissue engineering.

Original language	English
Pages (from-to)	88-95
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	110
DOIs	https://doi.org/10.1016/j.colsurfb.2013.04.016
Publication status	Published - Oct 1 2013
Externally published	Yes

Keywords

C2C12 Skeletal myoblast
Grooves
PLGA
RGD
YIGSR

ASJC Scopus subject areas

Biotechnology
Colloid and Surface Chemistry
Physical and Theoretical Chemistry
Surfaces and Interfaces

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10.1016/j.colsurfb.2013.04.016

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title = "Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering",

abstract = "Alignment of myocytes or myotubes is critical for skeletal muscle tissue engineering. In this study, grooved PLGA films (800. nm in width of ridge/groove and 600. nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for skeletal muscle tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for skeletal muscle tissue engineering.",

keywords = "C2C12 Skeletal myoblast, Grooves, PLGA, RGD, YIGSR",

author = "Wang, {Peng Yuan} and Wu, {Tsung Han} and Tsai, {Wei Bor} and Kuo, {Wei Hsuan} and Wang, {Meng Jiy}",

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T1 - Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering

AU - Wang, Peng Yuan

AU - Wu, Tsung Han

AU - Tsai, Wei Bor

AU - Kuo, Wei Hsuan

AU - Wang, Meng Jiy

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Alignment of myocytes or myotubes is critical for skeletal muscle tissue engineering. In this study, grooved PLGA films (800. nm in width of ridge/groove and 600. nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for skeletal muscle tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for skeletal muscle tissue engineering.

AB - Alignment of myocytes or myotubes is critical for skeletal muscle tissue engineering. In this study, grooved PLGA films (800. nm in width of ridge/groove and 600. nm in depth) incorporated with RGD or YIGSR peptides were fabricated to evaluate its efficacy for skeletal muscle tissue engineering. The growth and differentiation of C2C12 myoblasts were enhanced by the presentation of RGD or YIGSR compared with the untreated PLGA control. On the other hand, cell morphology was guided by the grooved structure, i.e. alignment of myoblasts and myotubes with the direction of grooves. This study elucidates the effects of both surface biochemical and topographic cues on the proliferation and differentiation of C2C12 myoblasts on biodegradable polymer films. Combination of surface topography and peptide presentation has a great potential in designing scaffolds for skeletal muscle tissue engineering.

KW - C2C12 Skeletal myoblast

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KW - RGD

KW - YIGSR

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ER -

Grooved PLGA films incorporated with RGD/YIGSR peptides for potential application on skeletal muscle tissue engineering

Abstract

Keywords

ASJC Scopus subject areas

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