Grape seed extract ameliorates tumor necrosis factor-α-induced inflammatory status of human umbilical vein endothelial cells

Chia Lun Chao, Nen Chung Chang, Ching Sung Weng, Kueir Rarn Lee, Shung Te Kao, Jiin Chyr Hsu, Feng Ming Ho

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Background: Inflammation has played a key role in the causation of atherosclerosis. However, the effects of grape seed extract (GSE) on the pro-inflammatory intracellular signaling, enzyme activity, and inflammatory mediators of endothelial cells have not been sufficiently studied, and less information exists on the comparison between GSE and vitamin C, a well-known antioxidant compound, on their anti-inflammatory properties. Purpose: We investigated the effects of GSE and vitamin C on the cell viability, oxidative stress, monocyte adhesion, the expression of nuclear factor-κB inhibitor (IκB), intercellular adhesion molecule-1 (ICAM-1) and cyclooxygenase-2 (COX-2), and the production of prostaglandin E 2 (PG E 2) in TNF-α-treated human umbilical vein endothelial cells (HUVECs). Methods: Cell viability was measured by MTT assay. The adhesion of THP-1 to HUVECs was evaluated by cell adhesion assay. The oxidized nucleoside 8- hydroxydeoxyguanosine (8-OHdG) (an indicator of oxidative damage to DNA), ICAM-1, and PG E 2 were measured by ELISA. IκB and COX-2 expression were evaluated by western blot analysis. Results: TNF-α (10, 20, and 50 ng/mL), GSE (50 and 200 μg/mL), or vitamin C (100 μM) did not affect cell viability. GSE (50-100 μg/mL) attenuated TNF-α (20 ng/mL)-induced 8-OHdG production, THP-1 adhesion, the expression of IκB degradation, ICAM-1 and COX-2, and the production of PGE 2 in a dose-dependent manner. Vitamin C (100 μM) also showed significant antioxidative and anti-inflammatory effects. Conclusions: GSE effectively ameliorates TNF-α-induced inflammatory status of HUVECs. The findings of the present study suggest that consumption of GSE may be beneficial to inflammatory atherosclerosis.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalEuropean Journal of Nutrition
Issue number6
Publication statusPublished - Sept 2011


  • Adhesion
  • Cyclooxygenase-2
  • Endothelial cell
  • Grape seed extract
  • Inflammation
  • Prostaglandin E

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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