Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Yin Shiou Lin; Wen Chung Wu; Shyr Yi Lin; Wen Chi Hou

doi:10.1007/s00726-014-1895-8

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Yin Shiou Lin, Wen Chung Wu, Shyr Yi Lin, Wen Chi Hou

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Among the eight amino acid hydroxamates tested, Glycine hydroxamate (GH) was the best inhibitor of mushroom tyrosinase (TYR). With l-tyrosine as substrate, the GH inhibition of the monophenolase activity of the mushroom TYR was noncompetitive. GH decreased not only TYR protein expression, but also melanin content, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia-associated transcription factor (MITF) expression in B16F10 melanoma cells while in the presence of α-melanocyte-stimulating hormone (α-MSH). GH also significantly decreased the isobutylmethylxanthine (IBMX)-induced increase in melanin content, which was not prevented by the ERK inhibitor PD98059. These results suggest GH has the potential for use in cosmetic hypopigmentation.

Original language	English
Pages (from-to)	617-625
Number of pages	9
Journal	Amino Acids
Volume	47
Issue number	3
DOIs	https://doi.org/10.1007/s00726-014-1895-8
Publication status	Published - Mar 2015

Keywords

Glycine hydroxamate
Hypopigmentation
Isobutylmethylxanthine
Melanin, microphthalmia-associated transcription factor
Protein kinase A
Tyrosinase

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1007/s00726-014-1895-8

Cite this

@article{625e7b62d73b487496e3ec1dc40fe924,

title = "Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways",

abstract = "Among the eight amino acid hydroxamates tested, Glycine hydroxamate (GH) was the best inhibitor of mushroom tyrosinase (TYR). With l-tyrosine as substrate, the GH inhibition of the monophenolase activity of the mushroom TYR was noncompetitive. GH decreased not only TYR protein expression, but also melanin content, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia-associated transcription factor (MITF) expression in B16F10 melanoma cells while in the presence of α-melanocyte-stimulating hormone (α-MSH). GH also significantly decreased the isobutylmethylxanthine (IBMX)-induced increase in melanin content, which was not prevented by the ERK inhibitor PD98059. These results suggest GH has the potential for use in cosmetic hypopigmentation.",

keywords = "Glycine hydroxamate, Hypopigmentation, Isobutylmethylxanthine, Melanin, microphthalmia-associated transcription factor, Protein kinase A, Tyrosinase",

author = "Lin, {Yin Shiou} and Wu, {Wen Chung} and Lin, {Shyr Yi} and Hou, {Wen Chi}",

note = "Publisher Copyright: {\textcopyright} 2014 Springer-Verlag Wien.",

year = "2015",

month = mar,

doi = "10.1007/s00726-014-1895-8",

language = "English",

volume = "47",

pages = "617--625",

journal = "Amino Acids",

issn = "0939-4451",

publisher = "Springer Wien",

number = "3",

}

TY - JOUR

T1 - Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

AU - Lin, Yin Shiou

AU - Wu, Wen Chung

AU - Lin, Shyr Yi

AU - Hou, Wen Chi

PY - 2015/3

Y1 - 2015/3

N2 - Among the eight amino acid hydroxamates tested, Glycine hydroxamate (GH) was the best inhibitor of mushroom tyrosinase (TYR). With l-tyrosine as substrate, the GH inhibition of the monophenolase activity of the mushroom TYR was noncompetitive. GH decreased not only TYR protein expression, but also melanin content, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia-associated transcription factor (MITF) expression in B16F10 melanoma cells while in the presence of α-melanocyte-stimulating hormone (α-MSH). GH also significantly decreased the isobutylmethylxanthine (IBMX)-induced increase in melanin content, which was not prevented by the ERK inhibitor PD98059. These results suggest GH has the potential for use in cosmetic hypopigmentation.

AB - Among the eight amino acid hydroxamates tested, Glycine hydroxamate (GH) was the best inhibitor of mushroom tyrosinase (TYR). With l-tyrosine as substrate, the GH inhibition of the monophenolase activity of the mushroom TYR was noncompetitive. GH decreased not only TYR protein expression, but also melanin content, tyrosinase-related protein (TRP)-1, TRP-2, and microphthalmia-associated transcription factor (MITF) expression in B16F10 melanoma cells while in the presence of α-melanocyte-stimulating hormone (α-MSH). GH also significantly decreased the isobutylmethylxanthine (IBMX)-induced increase in melanin content, which was not prevented by the ERK inhibitor PD98059. These results suggest GH has the potential for use in cosmetic hypopigmentation.

KW - Glycine hydroxamate

KW - Hypopigmentation

KW - Isobutylmethylxanthine

KW - Melanin, microphthalmia-associated transcription factor

KW - Protein kinase A

KW - Tyrosinase

UR - http://www.scopus.com/inward/record.url?scp=84925486580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925486580&partnerID=8YFLogxK

U2 - 10.1007/s00726-014-1895-8

DO - 10.1007/s00726-014-1895-8

M3 - Article

C2 - 25501504

AN - SCOPUS:84925486580

SN - 0939-4451

VL - 47

SP - 617

EP - 625

JO - Amino Acids

JF - Amino Acids

IS - 3

ER -

Glycine hydroxamate inhibits tyrosinase activity and melanin contents through downregulating cAMP/PKA signaling pathways

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this