TY - JOUR
T1 - Glutamine administration modulates endothelial progenitor cell and lung injury in septic mice
AU - Pai, Man Hui
AU - Shih, Yao Ming
AU - Shih, Juey Ming
AU - Yeh, Chiu Li
N1 - Publisher Copyright:
Copyright © 2016 by the Shock Society.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - ABSTRACT: This study investigated the effects of glutamine (GLN) administration on circulating endothelial progenitor cells (EPCs) and lung angiopoietin (Ang) gene expressions in polymicrobial sepsis. Mice were randomly assigned to a normal control group (NC), septic saline group (SS), and septic GLN group (SG). All mice were fed with a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The mice in SS group were injected with saline whereas SG group administered 0.75?g GLN/kg body weight once via tail vein 1?h after CLP. Mice were sacrificed 24, and 48?h post-CLP. Their blood and lungs were collected for further analysis. The results showed that, compared to normal mice, sepsis resulted in higher C-X-C motif chemokine-12, vascular endothelial growth factor, nitric oxide levels and a higher circulating EPC percentage. Also, inflammatory cytokine concentrations and Ang-2 gene expression were upregulated in lung tissues. GLN administration enhanced the mobilization of EPC, downregulated inflammatory cytokine production and the Ang-2 gene expressions in lungs. Histopathological findings showed that, the extent of inflammatory lesions of the lung alveolar was less severe in the SG group than the SS group after CLP. Our results suggest that a single dose of intravenous GLN administration after initiation of sepsis promote the mobilization of circulating EPC and modulates the balance of Ang-Tie2 axis that may improve the vascular function, ameliorate inflammation and protect lung injury against polymicrobial sepsis.
AB - ABSTRACT: This study investigated the effects of glutamine (GLN) administration on circulating endothelial progenitor cells (EPCs) and lung angiopoietin (Ang) gene expressions in polymicrobial sepsis. Mice were randomly assigned to a normal control group (NC), septic saline group (SS), and septic GLN group (SG). All mice were fed with a chow diet. Sepsis was induced by cecal ligation and puncture (CLP). The mice in SS group were injected with saline whereas SG group administered 0.75?g GLN/kg body weight once via tail vein 1?h after CLP. Mice were sacrificed 24, and 48?h post-CLP. Their blood and lungs were collected for further analysis. The results showed that, compared to normal mice, sepsis resulted in higher C-X-C motif chemokine-12, vascular endothelial growth factor, nitric oxide levels and a higher circulating EPC percentage. Also, inflammatory cytokine concentrations and Ang-2 gene expression were upregulated in lung tissues. GLN administration enhanced the mobilization of EPC, downregulated inflammatory cytokine production and the Ang-2 gene expressions in lungs. Histopathological findings showed that, the extent of inflammatory lesions of the lung alveolar was less severe in the SG group than the SS group after CLP. Our results suggest that a single dose of intravenous GLN administration after initiation of sepsis promote the mobilization of circulating EPC and modulates the balance of Ang-Tie2 axis that may improve the vascular function, ameliorate inflammation and protect lung injury against polymicrobial sepsis.
KW - Angiopoietin
KW - C-X-C chemokine receptor-12
KW - Endothelial progenitor cell
KW - Glutamine
KW - Sepsis
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U2 - 10.1097/SHK.0000000000000621
DO - 10.1097/SHK.0000000000000621
M3 - Article
C2 - 27058045
AN - SCOPUS:84964076468
SN - 1073-2322
VL - 46
SP - 587
EP - 592
JO - Shock
JF - Shock
IS - 5
ER -