Glucose-responsive nanogate of poly(methacrylic acid) brush-glucosamine network capping on the holes of chips for controlled insulin release

Feng Yen Lin, Bohr Ran Huang, Chun Yao Huang, Jem Kun Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Diabetes mellitus is a disease caused by deficient glycemic control by the pancreas that has caused serious health issues globally. Patients must inject insulin manually to stabilize their blood glucose levels (BGLs). Methods: A hole array was fabricated on a silicon wafer to graft poly(methacrylic acid) (PMAA) brushes from the silicon surface via atom transfer radical polymerization. Glucosamine (GA) was sequentially immobilized on the PMAA chains to obtain a P(MAA-GA) brush surrounding the hole edges on the silicon surface. After loading insulin inside the holes, biphenyl-4,4′-diboronic acid (DBA) was used to cross-link the P(MAA-GA) chains through the formation of boronate esters to cap the hole and block the release of insulin. Significant findings: The boronate esters were disassociated with glucose solution to open of the holes and the release of insulin, thus indicating a reversible association. The total amount of drug inside the chip was approximately 32.4 ± 1.8 g cm−2, which could be released at a rate of approximately 107 ± 5.7 ng h−1 cm−2 at a glucose concentration of 1 g L−1 for 270 h. Drug release was not significantly influenced by the presence of GA and amino acids, indicating excellent stability and selectivity of the chip.

Original languageEnglish
Article number104592
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume141
DOIs
Publication statusPublished - Dec 2022

Keywords

  • Glucose
  • Hole array
  • Insulin
  • PMAA brush
  • Subcutaneous implant chip

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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