Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma

Yu Jia Chang; Cheng Jeng Tai; Li Jen Kuo; Po Li Wei; Hung Hua Liang; Tsan Zon Liu; Weu Wang; Chen Jei Tai; Yuan Soon Ho; Chih Hsiung Wu; Ming Te Huang

doi:10.1245/s10434-011-1597-3

Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma

Yu Jia Chang, Cheng Jeng Tai, Li Jen Kuo, Po Li Wei, Hung Hua Liang, Tsan Zon Liu, Weu Wang, Chen Jei Tai, Yuan Soon Ho, Chih Hsiung Wu, Ming Te Huang

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

Original language	English
Pages (from-to)	2395-2403
Number of pages	9
Journal	Annals of Surgical Oncology
Volume	18
Issue number	8
DOIs	https://doi.org/10.1245/s10434-011-1597-3
Publication status	Published - Aug 2011

ASJC Scopus subject areas

Surgery
Oncology

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title = "Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma",

abstract = "Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.",

author = "Chang, {Yu Jia} and Tai, {Cheng Jeng} and Kuo, {Li Jen} and Wei, {Po Li} and Liang, {Hung Hua} and Liu, {Tsan Zon} and Weu Wang and Tai, {Chen Jei} and Ho, {Yuan Soon} and Wu, {Chih Hsiung} and Huang, {Ming Te}",

year = "2011",

month = aug,

doi = "10.1245/s10434-011-1597-3",

language = "English",

volume = "18",

pages = "2395--2403",

journal = "Annals of Surgical Oncology",

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T1 - Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma

AU - Chang, Yu Jia

AU - Tai, Cheng Jeng

AU - Kuo, Li Jen

AU - Wei, Po Li

AU - Liang, Hung Hua

AU - Liu, Tsan Zon

AU - Wang, Weu

AU - Tai, Chen Jei

AU - Ho, Yuan Soon

AU - Wu, Chih Hsiung

AU - Huang, Ming Te

PY - 2011/8

Y1 - 2011/8

N2 - Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

AB - Background: Glucose-regulated protein 78 (GRP78) plays an important role in the therapeutic treatment and progression of cancer. However, little is known about the effect of GRP78 expression to curcumin in hepatocellular carcinoma (HCC). Materials and Methods: In this study, we generated GRP78 knockdown cells (GRP78KD) by a short interfering RNA (siRNA) technique. The antiproliferation effects of curcumin were determined by MTT assay, TUNEL assay, and cell cycle determination. Results: We found that GRP78KD cells were more resistant to curcumin treatment compared with the parental cells in MTT assay. The apoptosis cell population was increased in scrambled-siRNA cells treated with curcumin compared with GRP78KD cells in cell cycle distribution and TUNEL assays. Finally, we found that knocking down GRP78 causes resistance to curcumin treatment through the suppression of caspase-3 and caspase-8 expression levels. Conclusions: We conclude that the expression level of GRP78 may contribute to the therapeutic effect of curcumin on HCC cells.

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JO - Annals of Surgical Oncology

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Glucose-regulated protein 78 (GRP78) mediated the efficacy to curcumin treatment on hepatocellular carcinoma

Abstract

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