Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 × 105 cells/ml/well) in M199 were incubated in 24-well culture plates at 37°C in a 5 % CO2 incubator. Ten μl of tested drugs with 1,10 and 100 μg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts of ginseng flowers (GF-1 ) with 10 to 100 μg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw <10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.
|Number of pages
|American Journal of Chinese Medicine
|Published - 2000
ASJC Scopus subject areas
- Complementary and alternative medicine