TY - JOUR
T1 - Giant polypeptides of skeletal muscle titin
T2 - Sedimentation equilibrium in guanidine hydrochloride
AU - Kurzban, Gary P.
AU - Wang, Kuan
N1 - Funding Information:
We thank Dr. P. Munk of the Departmento f Chemistry, University of Texas at Austin, for his advice and the use of the BeckmanM odel E ultracentrifuge. G.P.K. was supported in part by an NIH postdoctoral fellowship (CA 09182). This work was supported in part by U.S. Public Health Service grant DK 20270 to K.W., who is an American Heart Association Established Investigator.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988/2/15
Y1 - 1988/2/15
N2 - Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1 S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 × 106.
AB - Titin has been purified from rabbit skeletal myofibrils in guanidine hydrochloride by gel filtration on Sephacryl S-1000. The sedimentation coefficients of denatured titin in 6 M guanidine hydrochloride are highly concentration dependent and follow the equation: 1 S = 0.241 + 0.302 A280. Equilibrium sedimentation studies indicate that the average molecular weight of titin polypeptides is 2.4 to 2.6 × 106.
UR - http://www.scopus.com/inward/record.url?scp=0023872824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023872824&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(88)90750-4
DO - 10.1016/0006-291X(88)90750-4
M3 - Article
C2 - 3342063
AN - SCOPUS:0023872824
SN - 0006-291X
VL - 150
SP - 1155
EP - 1161
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -