Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan

W. L. Yu; S. C. Chen; S. W. Hung; Y. C. Chuang; J. G. Chung; I. C. Chen; L. T. Wu

doi:10.1111/j.1469-0691.2006.01488.x

Genetic association of bla_SHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan

W. L. Yu, S. C. Chen, S. W. Hung, Y. C. Chuang, J. G. Chung, I. C. Chen, L. T. Wu

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A cloned 5248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing bla_SHV-5 was sequenced. Insertion sequences IS26 and IS5 were found downstream from bla_SHV-5. The DNA sequences of the genetic environment surrounding bla_SHV-5 were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from bla_SHV-5, respectively. RecF may be involved in bla_SHV-5 translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan.

Original language	English
Pages (from-to)	806-809
Number of pages	4
Journal	Clinical Microbiology and Infection
Volume	12
Issue number	8
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01488.x
Publication status	Published - Aug 2006

Keywords

Bla gene
DNA sequence
Insertion sequence
Klebsiella pneumoniae
RecF
Sequencing

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1469-0691.2006.01488.x

Cite this

@article{902f84b4e1b8465f896c202804cab134,

title = "Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan",

abstract = "A cloned 5248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing blaSHV-5 was sequenced. Insertion sequences IS26 and IS5 were found downstream from blaSHV-5. The DNA sequences of the genetic environment surrounding blaSHV-5 were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from blaSHV-5, respectively. RecF may be involved in blaSHV-5 translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan.",

keywords = "Bla gene, DNA sequence, Insertion sequence, Klebsiella pneumoniae, RecF, Sequencing",

author = "Yu, {W. L.} and Chen, {S. C.} and Hung, {S. W.} and Chuang, {Y. C.} and Chung, {J. G.} and Chen, {I. C.} and Wu, {L. T.}",

note = "Funding Information: This work was financed in part by a grant CMU-93-M-11 from China Medical University and NSC 93-2314-B-384-004 from the National Science Council, the Executive Yuan, Taiwan. The nucleotide sequence reported in this work will appear under GenBank accession number AY518560.",

year = "2006",

month = aug,

doi = "10.1111/j.1469-0691.2006.01488.x",

language = "English",

volume = "12",

pages = "806--809",

journal = "Clinical Microbiology and Infection",

issn = "1198-743X",

publisher = "Elsevier B.V.",

number = "8",

}

TY - JOUR

T1 - Genetic association of blaSHV-5 with transposable elements IS26 and IS5 in Klebsiella pneumoniae from Taiwan

AU - Yu, W. L.

AU - Chen, S. C.

AU - Hung, S. W.

AU - Chuang, Y. C.

AU - Chung, J. G.

AU - Chen, I. C.

AU - Wu, L. T.

N1 - Funding Information: This work was financed in part by a grant CMU-93-M-11 from China Medical University and NSC 93-2314-B-384-004 from the National Science Council, the Executive Yuan, Taiwan. The nucleotide sequence reported in this work will appear under GenBank accession number AY518560.

PY - 2006/8

Y1 - 2006/8

N2 - A cloned 5248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing blaSHV-5 was sequenced. Insertion sequences IS26 and IS5 were found downstream from blaSHV-5. The DNA sequences of the genetic environment surrounding blaSHV-5 were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from blaSHV-5, respectively. RecF may be involved in blaSHV-5 translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan.

AB - A cloned 5248-bp EcoRI fragment from the Klebsiella pneumoniae transferable plasmid pKP53 (> 70 kb) containing blaSHV-5 was sequenced. Insertion sequences IS26 and IS5 were found downstream from blaSHV-5. The DNA sequences of the genetic environment surrounding blaSHV-5 were homologous to plasmid p1658/97 from Escherichia coli, containing a truncated recF gene and a truncated deoR gene upstream and downstream from blaSHV-5, respectively. RecF may be involved in blaSHV-5 translocation to the plasmid by RecF-dependent recombination. This novel genetic environment may be associated with the successful proliferation and/or expression of SHV-5 in K. pneumoniae strains from Taiwan.

KW - Bla gene

KW - DNA sequence

KW - Insertion sequence

KW - Klebsiella pneumoniae

KW - RecF

KW - Sequencing

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