TY - JOUR
T1 - Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells
AU - Chang, Hsi
AU - Yoshimoto, Momoko
AU - Umeda, Katsutsugu
AU - Iwasa, Toru
AU - Mizuno, Yuta
AU - Fukada, So Ichiro
AU - Yamamoto, Hiroshi
AU - Motohashi, Norio
AU - Yuko-Miyagoe-Suzuki,
AU - Takeda, Shin'ichi
AU - Heike, Toshio
AU - Nakahata, Tatsutoshi
PY - 2009/6
Y1 - 2009/6
N2 - Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7-positive satellite-like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel-coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7-positive satellite-like cells were enriched by fluorescence-activated cell sorting using a novel anti-satellite cell antibody, SM/C-2.6. SM/C-2.6-positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo. Furthermore, the cells demonstrate satellite cell characteristics such as extensive self-renewal capacity in subsequent muscle injury model, long-term engraftment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite-like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disorders.
AB - Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7-positive satellite-like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel-coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7-positive satellite-like cells were enriched by fluorescence-activated cell sorting using a novel anti-satellite cell antibody, SM/C-2.6. SM/C-2.6-positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo. Furthermore, the cells demonstrate satellite cell characteristics such as extensive self-renewal capacity in subsequent muscle injury model, long-term engraftment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite-like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disorders.
KW - High engraftment efficiency
KW - Long-term engraftment
KW - Secondary transplantation
KW - Self-renewal
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U2 - 10.1096/fj.08-123661
DO - 10.1096/fj.08-123661
M3 - Article
C2 - 19168704
AN - SCOPUS:67649311948
SN - 0892-6638
VL - 23
SP - 1907
EP - 1919
JO - FASEB Journal
JF - FASEB Journal
IS - 6
ER -