Generation of transplantable, functional satellite-like cells from mouse embryonic stem cells

Hsi Chang, Momoko Yoshimoto, Katsutsugu Umeda, Toru Iwasa, Yuta Mizuno, So Ichiro Fukada, Hiroshi Yamamoto, Norio Motohashi, Yuko-Miyagoe-Suzuki, Shin'ichi Takeda, Toshio Heike, Tatsutoshi Nakahata

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)


Satellite cells are myogenic stem cells responsible for the postnatal regeneration of skeletal muscle. Here we report the successful in vitro induction of Pax7-positive satellite-like cells from mouse embryonic stem (mES) cells. Embryoid bodies were generated from mES cells and cultured on Matrigel-coated dishes with Dulbecco's modified Eagle medium containing fetal bovine serum and horse serum. Pax7-positive satellite-like cells were enriched by fluorescence-activated cell sorting using a novel anti-satellite cell antibody, SM/C-2.6. SM/C-2.6-positive cells efficiently differentiate into skeletal muscle fibers both in vitro and in vivo. Furthermore, the cells demonstrate satellite cell characteristics such as extensive self-renewal capacity in subsequent muscle injury model, long-term engraftment up to 24 wk, and the ability to be secondarily transplanted with remarkably high engraftment efficiency compared to myoblast transplantation. This is the first report of transplantable, functional satellite-like cells derived from mES cells and will provide a foundation for new therapies for degenerative muscle disorders.

Original languageEnglish
Pages (from-to)1907-1919
Number of pages13
JournalFASEB Journal
Issue number6
Publication statusPublished - Jun 2009
Externally publishedYes


  • High engraftment efficiency
  • Long-term engraftment
  • Secondary transplantation
  • Self-renewal

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry
  • Biotechnology


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