Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

Yuta Mizuno; Hsi Chang; Katsutsugu Umeda; Akira Niwa; Toru Iwasa; Tomonari Awaya; So Ichiro Fukada; Hiroshi Yamamoto; Shinya Yamanaka; Tatsutoshi Nakahata; Toshio Heike

doi:10.1096/fj.09-137174

Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

Yuta Mizuno
, Hsi Chang
, Katsutsugu Umeda
, Akira Niwa
, Toru Iwasa
, Tomonari Awaya
, So Ichiro Fukada
, Hiroshi Yamamoto
, Shinya Yamanaka
, Tatsutoshi Nakahata
, Toshio Heike

Research output: Contribution to journal › Article › peer-review

145 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6⁺ cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.

Original language	English
Pages (from-to)	2245-2253
Number of pages	9
Journal	FASEB Journal
Volume	24
Issue number	7
DOIs	https://doi.org/10.1096/fj.09-137174
Publication status	Published - Jul 2010
Externally published	Yes

Keywords

Duchenne muscular dystrophy
High engraftment efficiency
Long-term engraftment
No teratoma formation
Pax7

ASJC Scopus subject areas

Genetics
Molecular Biology
Biochemistry
Biotechnology

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10.1096/fj.09-137174

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title = "Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells",

abstract = "Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6+ cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.",

keywords = "Duchenne muscular dystrophy, High engraftment efficiency, Long-term engraftment, No teratoma formation, Pax7",

author = "Yuta Mizuno and Hsi Chang and Katsutsugu Umeda and Akira Niwa and Toru Iwasa and Tomonari Awaya and Fukada, \{So Ichiro\} and Hiroshi Yamamoto and Shinya Yamanaka and Tatsutoshi Nakahata and Toshio Heike",

year = "2010",

month = jul,

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language = "English",

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AU - Mizuno, Yuta

AU - Chang, Hsi

AU - Umeda, Katsutsugu

AU - Niwa, Akira

AU - Iwasa, Toru

AU - Awaya, Tomonari

AU - Fukada, So Ichiro

AU - Yamamoto, Hiroshi

AU - Yamanaka, Shinya

AU - Nakahata, Tatsutoshi

AU - Heike, Toshio

PY - 2010/7

Y1 - 2010/7

N2 - Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6+ cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.

AB - Induced pluripotent stem (iPS) cells, which are a type of pluripotent stem cell generated from reprogrammed somatic cells, are expected to have potential for patient-oriented disease investigation, drug screening, toxicity tests, and transplantation therapies. Here, we demonstrated that murine iPS cells have the potential to develop in vitro into skeletal muscle stem/progenitor cells, which are almost equivalent to murine embryonic stem cells. Cells with strong in vitro myogenic potential effectively were enriched by fluorescence-activated cell sorting using the anti-satellite cell antibody SM/C-2.6. Furthermore, on transplantation into mdx mice, SM/C-2.6+ cells exerted sustained myogenic lineage differentiation in injured muscles, while providing long-lived muscle stem cell support. Our data suggest that iPS cells have the potential to be used in clinical treatment of muscular dystrophies.

KW - Duchenne muscular dystrophy

KW - High engraftment efficiency

KW - Long-term engraftment

KW - No teratoma formation

KW - Pax7

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AN - SCOPUS:77954448510

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VL - 24

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JO - FASEB Journal

JF - FASEB Journal

IS - 7

ER -

Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells

Abstract

Keywords

ASJC Scopus subject areas

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