Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells

Pan Yueh; Chiu Ya-Hsu; Chiu Shao-Chih; Cho Der-Yang; Lee Liang-Ming; Wen Yu-Ching; Whang Peng Jacqueline; Hsiao Chi-Hao; Shih Ping-Hsiao

doi:10.21873/anticanres.14525

Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells

Pan Yueh, Chiu Ya-Hsu, Chiu Shao-Chih, Cho Der-Yang, Lee Liang-Ming, Wen Yu-Ching, Whang Peng Jacqueline, Hsiao Chi-Hao, Shih Ping-Hsiao

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Background/Aim: Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. Materials and Methods: The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. Results: After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which

Original language	English
Pages (from-to)	5221-5227
Number of pages	7
Journal	Anticancer Research
Volume	40
Issue number	9
DOIs	https://doi.org/10.21873/anticanres.14525
Publication status	Published - Sept 2020

Keywords

Bladder cancer
Chemotherapy
Cytotoxicity
Gamma/delta T-cell
Immunocellular therapy

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.14525

Cite this

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title = "Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells",

abstract = "Background/Aim: Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. Materials and Methods: The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. Results: After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which",

keywords = "Bladder cancer, Chemotherapy, Cytotoxicity, Gamma/delta T-cell, Immunocellular therapy",

author = "Pan Yueh and Chiu Ya-Hsu and Chiu Shao-Chih and Cho Der-Yang and Lee Liang-Ming and Wen Yu-Ching and Jacqueline, {Whang Peng} and Hsiao Chi-Hao and Shih Ping-Hsiao",

note = "Funding Information: This work was supported by the Department of Medical Research, China Medical University Hospital (grant nos. DMR-107-153 and DMR-CELL-1809) and Taipei Municipal WanFang Hospital (grant no.109-wf-eva-28). Publisher Copyright: {\textcopyright} 2020 International Institute of Anticancer Research. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = sep,

doi = "10.21873/anticanres.14525",

language = "English",

volume = "40",

pages = "5221--5227",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

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}

TY - JOUR

T1 - Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells

AU - Yueh, Pan

AU - Ya-Hsu, Chiu

AU - Shao-Chih, Chiu

AU - Der-Yang, Cho

AU - Liang-Ming, Lee

AU - Yu-Ching, Wen

AU - Jacqueline, Whang Peng

AU - Chi-Hao, Hsiao

AU - Ping-Hsiao, Shih

N1 - Funding Information: This work was supported by the Department of Medical Research, China Medical University Hospital (grant nos. DMR-107-153 and DMR-CELL-1809) and Taipei Municipal WanFang Hospital (grant no.109-wf-eva-28). Publisher Copyright: © 2020 International Institute of Anticancer Research. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/9

Y1 - 2020/9

N2 - Background/Aim: Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. Materials and Methods: The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. Results: After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which

AB - Background/Aim: Bladder cancer (BLCA, urothelial bladder cancer) is one of the most common malignancies with increasing incidence and mortality worldwide. Poor diagnosis and the limitation of treatment is still an unmet need in clinical practice. γδ T-Cells have been paid increasing attention because of their potent cytotoxicity against tumors. Herein, we investigated the cytolytic effect of γδ T-cells in combination with the chemotherapeutic drug, carboplatin, against BLCA cells. Materials and Methods: The standard protocol for the induction and expansion of peripheral blood mononuclear cell-derived γδ T-cells was a zoledronic acid/interleukin-2-based medium system for 2 weeks. The cytotoxicity of γδ T-cells with and without carboplatin against BLCA cells was examined. Results: After incubation, T-cell receptor-positive γδ T-cells showed a natural killer cell-like phenotypic characteristic and dose-dependently increased cytotoxicity against BLCA cells. Interestingly, we found that in advanced BLCA cells, which

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KW - Chemotherapy

KW - Cytotoxicity

KW - Gamma/delta T-cell

KW - Immunocellular therapy

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Gamma/delta T-cells enhance carboplatin-induced cytotoxicity towards advanced bladder cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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