TY - JOUR
T1 - Functional role of matrix metalloproteinase-28 in the oral squamous cell carcinoma
AU - Lin, Mei Huei
AU - Liu, Shyun Yeu
AU - Su, Hsiao Jing
AU - Liu, Young Chau
PY - 2006/10
Y1 - 2006/10
N2 - The newly identified MMP-28 has been shown to be expressed in several types of carcinomas, however, its functional role in transformation events is unknown. This study was to assess whether this proteinase plays a role in oral tumor malignancy. By using RT-PCR, we found that expression of MMP-28 was significantly higher in 92 oral squamous cell carcinomas (OSCCs) (52/92, 56.5%) than in seven oral premalignant lesions (OPMLs) (0/7, 0%) (P = 0.004). No statistically significant correlation was found between MMP-28 expression and tumor stage, thickness, size, and metastasis. Both mRNA and protein of MMP-28 were preferentially concentrated in OSCC specimens than in neighboring tissues as analyzed by semi-quantitative RT-PCR (P = 0.015) and immunohistochemistry, respectively. Transfection of OSCC and esophageal carcinoma cell lines with MMP-28 antisense oligodeoxynucleotide (AODN) resulted in the reduced secretion of MMP-28 protein and the ability of colony formation in soft agar without affecting cell growth. Our findings show the close correlation between MMP-28 and OSCC, and support a role for MMP-28 in the anchorage-independent growth of both OSCC and esophageal carcinomas.
AB - The newly identified MMP-28 has been shown to be expressed in several types of carcinomas, however, its functional role in transformation events is unknown. This study was to assess whether this proteinase plays a role in oral tumor malignancy. By using RT-PCR, we found that expression of MMP-28 was significantly higher in 92 oral squamous cell carcinomas (OSCCs) (52/92, 56.5%) than in seven oral premalignant lesions (OPMLs) (0/7, 0%) (P = 0.004). No statistically significant correlation was found between MMP-28 expression and tumor stage, thickness, size, and metastasis. Both mRNA and protein of MMP-28 were preferentially concentrated in OSCC specimens than in neighboring tissues as analyzed by semi-quantitative RT-PCR (P = 0.015) and immunohistochemistry, respectively. Transfection of OSCC and esophageal carcinoma cell lines with MMP-28 antisense oligodeoxynucleotide (AODN) resulted in the reduced secretion of MMP-28 protein and the ability of colony formation in soft agar without affecting cell growth. Our findings show the close correlation between MMP-28 and OSCC, and support a role for MMP-28 in the anchorage-independent growth of both OSCC and esophageal carcinomas.
KW - Colony formation
KW - Esophageal carcinoma
KW - Matrix metalloproteinase
KW - MMP-28
KW - Oral squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=33748902434&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748902434&partnerID=8YFLogxK
U2 - 10.1016/j.oraloncology.2005.12.012
DO - 10.1016/j.oraloncology.2005.12.012
M3 - Article
C2 - 16730219
AN - SCOPUS:33748902434
SN - 1368-8375
VL - 42
SP - 907
EP - 913
JO - Oral Oncology
JF - Oral Oncology
IS - 9
ER -