Functional effects of let-7g expression in colon cancer metastasis

Che Mai Chang; Henry Sung Ching Wong; Chien Yu Huang; Wen Li Hsu; Zhi Feng Maio; Siou Jin Chiu; Yao Ting Tsai; Ben Kuen Chen; Yu Jui Yvonne Wan; Jaw Yuan Wang; Wei Chiao Chang

doi:10.3390/cancers11040489

Functional effects of let-7g expression in colon cancer metastasis

Che Mai Chang, Henry Sung Ching Wong, Chien Yu Huang, Wen Li Hsu, Zhi Feng Maio, Siou Jin Chiu, Yao Ting Tsai, Ben Kuen Chen, Yu Jui Yvonne Wan, Jaw Yuan Wang, Wei Chiao Chang

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

Original language	English
Article number	489
Journal	Cancers
Volume	11
Issue number	4
DOIs	https://doi.org/10.3390/cancers11040489
Publication status	Published - Apr 1 2019

Keywords

Colorectal cancer
Let-7g
MicroRNA
Store-operated calcium (SOC)
The cancer genome atlas (TCGA)

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/cancers11040489

Cite this

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title = "Functional effects of let-7g expression in colon cancer metastasis",

abstract = "MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.",

keywords = "Colorectal cancer, Let-7g, MicroRNA, Store-operated calcium (SOC), The cancer genome atlas (TCGA)",

author = "Chang, {Che Mai} and Wong, {Henry Sung Ching} and Huang, {Chien Yu} and Hsu, {Wen Li} and Maio, {Zhi Feng} and Chiu, {Siou Jin} and Tsai, {Yao Ting} and Chen, {Ben Kuen} and Wan, {Yu Jui Yvonne} and Wang, {Jaw Yuan} and Chang, {Wei Chiao}",

note = "Funding Information: Funding: This work was supported by grants through funding from the Ministry of Science and Technology (MOST108-2321-B-037-001), the Kaohsiung Medical University Hospital (KMUHS10701, KMUHS10706) and Taipei Medical University (106-5807-001-400). Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = apr,

day = "1",

doi = "10.3390/cancers11040489",

language = "English",

volume = "11",

journal = "Cancers",

issn = "2072-6694",

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TY - JOUR

T1 - Functional effects of let-7g expression in colon cancer metastasis

AU - Chang, Che Mai

AU - Wong, Henry Sung Ching

AU - Huang, Chien Yu

AU - Hsu, Wen Li

AU - Maio, Zhi Feng

AU - Chiu, Siou Jin

AU - Tsai, Yao Ting

AU - Chen, Ben Kuen

AU - Wan, Yu Jui Yvonne

AU - Wang, Jaw Yuan

AU - Chang, Wei Chiao

N1 - Funding Information: Funding: This work was supported by grants through funding from the Ministry of Science and Technology (MOST108-2321-B-037-001), the Kaohsiung Medical University Hospital (KMUHS10701, KMUHS10706) and Taipei Medical University (106-5807-001-400). Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

AB - MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment.

KW - Colorectal cancer

KW - Let-7g

KW - MicroRNA

KW - Store-operated calcium (SOC)

KW - The cancer genome atlas (TCGA)

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M1 - 489

ER -

Functional effects of let-7g expression in colon cancer metastasis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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